Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 24, Problem 4P
Interpretation Introduction
Interpretation:
A maximal separation between the malonyl transferase and the β-ketoacyl-ACP synthase active sites should be estimated.
Concept Introduction:
Fatty acid synthase is a multi-enzyme protein which catalyzes the fatty acid biosynthesis.Major function is catalyzing the formation of palmitate from acetyl CoA and malonyl CoA. Mammalian fatty acid synthase contains two identical protein subunits. Three catalytic domains are in N-terminal section which are β-ketoacyl-ACP synthase, malonyl/acetyl transferase and dehydrase. Four catalytic domains are in C-terminal section, which are enoyl reductase, ketoacyl reductase, acyl carrier protein and thioesterase. N terminal section and C terminal section is separated by 600 residues.
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M-CSA Mechanism and Catalytic Site Atlas (ebi.ac.uk)
(ii)
Acyl Carrier Protein S-acetyltransferase (EC 2.3.1.38) is a transferase enzyme that
catalyzes the first biosynthetic pathway for fatty acid synthase. It transfers the acyl
group (CH3CO) first from coenzyme A to a cysteine residue in the active site. This is
similar to what happens in Chymotrypsin, however utilizing a sulfur instead of an
oxygen. The acyl group is then transferred to the molecule ACP. Provide the enzyme-
catalyzed mechanism for the reaction below, making sure to identify the roles of all key
amino acids:
i
H3C
SCOA
acetyl COA
enzyme
+ HS
i
H3C
SACP
acetyl ACP
Biosynthesis of fatty acid 20:D6 from acetyl-CoA occurs in the __________ of mammalian cells.
Cytosol
Endoplasmic reticulum
Both A and B
Neither A nor B
Assuming all three carbon atoms of glycerol are labeled as C14 radioisotopes and the radioisotope-labeled glycerol undergoes metabolism in animals. All of the following molecules in the animal maycontain C14 radioisotopes EXCEPT:
Pyruvate
Acetyl-CoA
Glutamine
Fatty acids
Oxaloacetate
All of the above molecules may contain C14 radioisotope
Pls help due asap
Chapter 24 Solutions
Biochemistry
Ch. 24 - Explaining the Stoichiometry of Fatty Acid...Ch. 24 - Tracing Carbon Atom Incorporation in Fatty Acids...Ch. 24 - Modeling the Regulation of AcetyI-CoA Carboxylase...Ch. 24 - Prob. 4PCh. 24 - Prob. 5PCh. 24 - Prob. 6PCh. 24 - Understanding the Stoichiometry of Cholesterol...Ch. 24 - Prob. 8PCh. 24 - Prob. 9PCh. 24 - Prob. 10P
Ch. 24 - Prob. 11PCh. 24 - Understanding the Mechanism of the...Ch. 24 - Prob. 13PCh. 24 - Understanding the Mechanism of the HMC-CoA...Ch. 24 - Prob. 15PCh. 24 - Prob. 16PCh. 24 - Prob. 17PCh. 24 - Prob. 18PCh. 24 - Prob. 19PCh. 24 - Prob. 20PCh. 24 - Prob. 21PCh. 24 - Prob. 22PCh. 24 - Prob. 23PCh. 24 - Prob. 24PCh. 24 - Prob. 25P
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