A series of steps or a cycle of reactions that could achieve the enrichment of phosphatidylinositol with 1-stearoyl-2-archidonoyl phosphatidylinositol needs to be proposed. Concept Introduction: The phosphatidylinositol cycle involves in cell signaling. Its major purpose is to synthesize phosphatidylinositol and its phosphorylated forms. The lipid intermediate of PI cycle are highly enriched with 1-stearoyl-2-arachidnoyl molecular species. This enrichment is retained as long as the intermediates are segregated from other lipids. What happens in the enrichment of a particular phospholipid molecule is selective addition of one specific type of alkyl or the acyl chain. There are several enzymes that involve in the conversion of phospholipids to exhibit specific acyl or alkyl chains.

Question

The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

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Question

Chapter 24, Problem 20P

Interpretation Introduction

Interpretation:

A series of steps or a cycle of reactions that could achieve the enrichment of phosphatidylinositol with 1-stearoyl-2-archidonoyl phosphatidylinositol needs to be proposed.

Concept Introduction:

The phosphatidylinositol cycle involves in cell signaling. Its major purpose is to synthesize phosphatidylinositol and its phosphorylated forms. The lipid intermediate of PI cycle are highly enriched with 1-stearoyl-2-arachidnoyl molecular species. This enrichment is retained as long as the intermediates are segregated from other lipids. What happens in the enrichment of a particular phospholipid molecule is selective addition of one specific type of alkyl or the acyl chain. There are several enzymes that involve in the conversion of phospholipids to exhibit specific acyl or alkyl chains.

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!