Biochemistry
Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 24, Problem 16P
Interpretation Introduction

Interpretation:

Examination required for the mammalian FAS structure and determination for the distance between several functional sites.

Concept introduction:

FAS i.e. Fatty acid synthetase is a multi-enzyme protein which catalyzes fatty acid synthesis.

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You’ve isolated a protein and determined that the Native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD.  The enzyme was found to be 0.829% NAD (by weight). What further can be said regarding the structure of the polypeptide?
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