Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 24, Problem 18P
Interpretation Introduction
Interpretation:
How the study of LDL receptor binding by a human rhinovirus HRV2, provided support for model of LDL particle displacement should be discussed.
Concept Introduction:
LDL receptor has five domains. N terminal LDL binding domain contains seven cysteine rich repeats referred as R1 to R7. Next epidermal growth factor repeats and ß propeller module can be found. Those segments are followed by O-linked oligosaccharide, a membrane spanning segment and another segment extended towards the cytosol. LDL particles bind with R4 and R5 cysteine repeats. At low pH, the LDL receptor polypeptide folds so that ß propeller domain is associated with R4 and R5 displacing the LDL particle. These two repeats contain two loops connected by 3 disulfide bonds. Second loop of each repeat have Asp and Glu acidic residues and acts as a Ca2+ binding site.
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An active site of a hypothetical serine protease with a peptide substrate bound is shown below:
This serine protease has 3 specificity pockets (S1, S2, S1') as shown in the figure above. S1 pocket has a glutamic acid in the bottom, the S2 pocket is small and hydrophobic, and the S1' pocket is deep and hydrophobic.
Based on this information you can conclude that R1 is most likely side chain of
a. Asp
b. Lys
c. Lle
d. Gly
e. Phe
Shown below is a schematic diagram of the E. coli leader peptidase (Lep), which has several basic amino acids in a cytoplasmic loop. Propose a mutant of Lep that would be a test of the “inside positive” rule for the orientation of proteins in membranes.
Chapter 24 Solutions
Biochemistry
Ch. 24 - Explaining the Stoichiometry of Fatty Acid...Ch. 24 - Tracing Carbon Atom Incorporation in Fatty Acids...Ch. 24 - Modeling the Regulation of AcetyI-CoA Carboxylase...Ch. 24 - Prob. 4PCh. 24 - Prob. 5PCh. 24 - Prob. 6PCh. 24 - Understanding the Stoichiometry of Cholesterol...Ch. 24 - Prob. 8PCh. 24 - Prob. 9PCh. 24 - Prob. 10P
Ch. 24 - Prob. 11PCh. 24 - Understanding the Mechanism of the...Ch. 24 - Prob. 13PCh. 24 - Understanding the Mechanism of the HMC-CoA...Ch. 24 - Prob. 15PCh. 24 - Prob. 16PCh. 24 - Prob. 17PCh. 24 - Prob. 18PCh. 24 - Prob. 19PCh. 24 - Prob. 20PCh. 24 - Prob. 21PCh. 24 - Prob. 22PCh. 24 - Prob. 23PCh. 24 - Prob. 24PCh. 24 - Prob. 25P
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