1/V (min/uM) 0.12 Kinetic Analysis of Enzyme Z 0.10 0.08 0.06 0.04 0.02- y = 0.224x+0.0224 0.00 -0.15 -0.10 -0.05 0.00 0.05 0.10 0.15 0.20 0.25 0.30 0.35 0.40 1/[S] (UM-1) a. Give the equation that was used to plot the line. Which are the x and y terms in this equation? b. Use the graph to calculate KM and Vmax for Enzyme Z. c. We discussed three types of reversible inhibition in class. How would these be kinetically distinguishable in an experiment like the one given here? Use the graph to show what would be observed (draw and label the lines).
1/V (min/uM) 0.12 Kinetic Analysis of Enzyme Z 0.10 0.08 0.06 0.04 0.02- y = 0.224x+0.0224 0.00 -0.15 -0.10 -0.05 0.00 0.05 0.10 0.15 0.20 0.25 0.30 0.35 0.40 1/[S] (UM-1) a. Give the equation that was used to plot the line. Which are the x and y terms in this equation? b. Use the graph to calculate KM and Vmax for Enzyme Z. c. We discussed three types of reversible inhibition in class. How would these be kinetically distinguishable in an experiment like the one given here? Use the graph to show what would be observed (draw and label the lines).
Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter5: Biomedical Electronics: Digital
Section: Chapter Questions
Problem 3P
Related questions
Question
100%
use the line weaver burk plot to answer the questions
please answer all wues
![1/V (min/uM)
0.12
Kinetic Analysis of Enzyme Z
0.10
0.08
0.06
0.04
0.02-
y = 0.224x+0.0224
0.00
-0.15
-0.10 -0.05 0.00
0.05
0.10
0.15
0.20
0.25
0.30
0.35
0.40
1/[S] (UM-1)
a. Give the equation that was used to plot the line. Which are the x and y terms in this
equation?
b. Use the graph to calculate KM and Vmax for Enzyme Z.
c. We discussed three types of reversible inhibition in class. How would these be kinetically
distinguishable in an experiment like the one given here? Use the graph to show what would be
observed (draw and label the lines).](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F250eaf95-2834-4096-98aa-2dd423e2be3c%2F3f2e31c4-9ff9-453a-9b69-25f02d99b64f%2F28vffqi_processed.jpeg&w=3840&q=75)
Transcribed Image Text:1/V (min/uM)
0.12
Kinetic Analysis of Enzyme Z
0.10
0.08
0.06
0.04
0.02-
y = 0.224x+0.0224
0.00
-0.15
-0.10 -0.05 0.00
0.05
0.10
0.15
0.20
0.25
0.30
0.35
0.40
1/[S] (UM-1)
a. Give the equation that was used to plot the line. Which are the x and y terms in this
equation?
b. Use the graph to calculate KM and Vmax for Enzyme Z.
c. We discussed three types of reversible inhibition in class. How would these be kinetically
distinguishable in an experiment like the one given here? Use the graph to show what would be
observed (draw and label the lines).
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 2 steps with 8 images
Recommended textbooks for you
Essentials of Pharmacology for Health Professions
Nursing
ISBN:
9781305441620
Author:
WOODROW
Publisher:
Cengage
Essentials of Pharmacology for Health Professions
Nursing
ISBN:
9781305441620
Author:
WOODROW
Publisher:
Cengage
Essentials Health Info Management Principles/Prac…
Health & Nutrition
ISBN:
9780357191651
Author:
Bowie
Publisher:
Cengage
Human Heredity: Principles and Issues (MindTap Co…
Biology
ISBN:
9781305251052
Author:
Michael Cummings
Publisher:
Cengage Learning