Chapter 10, Problem 10QP

1. b. Compounds A, B, C, and D are known to be intermediates in the pathway for production of protein E. To determine where the block in protein-E production occurred in each individual, the various intermediates were given to each individual’s cel Is in culture. After a few weeks of growth with the intermediate, the cells were assayed for the production of protein E. The results for each individual’s cells are given in the following table. A plus sign means that protein E was produced after the cells were given the intermediate listed at the top of the column. A minus sign means that the cells still could not produce protein E even after being exposed to the intermediate at the top of the column.

Denote the point in the pathway in which each individual is blocked.