The following questions are based on information given below: Puc is a transcriptional target of the JNK signaling pathway. Therefore, the level of Puc-LacZ reporter can be used to monitor JNK signaling activity. Ubi-GFP is a construct with GFP expression under the control of a ubiquitously expressed promoter. To study the effect of loss of dmyc function during wing disc development, you used the dmycP0, which is a partial loss of function allele, and generated the following genotype flies: dmycP0FRT19 / Ubi-GFP FRT19; +; Puc-lacZ / Hs-FLP. After heat shock to induce mutant clones, you examined wing discs for GFP and Puc-LacZ levels (shown in green and red in the images below, respectively) 1. Identify and mark the cells that are homozygous wild type, heterozygous, and homozygous for the dmycP0 mutation in image A. 1. What effect does loss of dmyc have on JNK signaling levels? Is this effect cell autonomous?
The following questions are based on information given below:
Puc is a transcriptional target of the JNK signaling pathway. Therefore, the level of Puc-LacZ reporter can be used to monitor JNK signaling activity. Ubi-GFP is a construct with GFP expression under the control of a ubiquitously expressed promoter. To study the effect of loss of dmyc function during wing disc development, you used the dmycP0, which is a partial loss of function allele, and generated the following genotype flies: dmycP0FRT19 / Ubi-GFP FRT19; +; Puc-lacZ / Hs-FLP.
After heat shock to induce mutant clones, you examined wing discs for GFP and Puc-LacZ levels (shown in green and red in the images below, respectively)
1. Identify and mark the cells that are homozygous wild type, heterozygous, and homozygous for the dmycP0 mutation in image A.
1. What effect does loss of dmyc have on JNK signaling levels? Is this effect cell autonomous?
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