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Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Puc is a transcriptional target of the JNK signaling pathway. Therefore, the level of Puc-LacZ reporter can be used to monitor JNK signaling activity. Ubi-GFP is a construct with GFP expression under the control of a ubiquitously expressed promoter. To study the effect of loss of dmyc function during wing disc development, you used the dmyc^P0, which is a partial loss of function allele, and generated the following genotype flies: dmyc^P0FRT19 / Ubi-GFP FRT19; +; Puc-lacZ / Hs-FLP.

After heat shock to induce mutant clones, you examined wing discs for GFP and Puc-LacZ levels (shown in green and red in the images below, respectively)

1. Identify and mark the cells that are homozygous wild type, heterozygous, and homozygous for the dmyc^P0 mutation in image A.

1. What effect does loss of dmyc have on JNK signaling levels? Is this effect cell autonomous?

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