You study the expression of the hexose kinase gene and capture the following electron micrograph of the gene being expressed. MRNA 1 20 ORI 40 60 TCGAG ATACGCGATGATATTACTGGIAATATGGGGATGCACTATC 3' ТTCGAGCTCТСGTCGTCO 5' AAGCTCGAGAGCAGCAGCTCTАTGCGCTАСТАТААTGACCAТТАТАCЕССТАСGTGATAG RNA promoter polymerase Practice Question 4 E) You also study the expression of different mutants for this gene. Mutant A has a single base pair substitution with the C/G being replaced with G/C base pair at position 30 (position denoted by the * in the sequence above). For mutant A answer the following: Does this mutation change the sequence of the protein produced? Why or why not? If it does change the sequence of protein be sure to write out the new sequence. If it does not change the protein sequence, what effect (if any) would you expect it to have on expression of the gene? 1 20 ORI 40 60 3'..TTCGAGCTCTCGTCGTCGAGATACGCGATGATATTACTGGTAATATGGGGATGCACTATC...5' 5'..AAGCTCGAGAGCAGCAGCTCTATGCGCTACTATAATGACCATTATACCCCTACGTGATAG...3' promoter ribosome

Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter15: Genomes And Genomics
Section: Chapter Questions
Problem 13QP
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You study the expression of the hexose kinase gene and capture the following electron micrograph of
the gene being expressed.
mRNA
20
ORI
40
60
3'
GATATTACTGGI
АAGCTCGAGAGCAGCAGCTCТАTGCGCTACТАТААТGACCАТТАТАСЕССТАCGTGATAG
TTCGAGCTCTCGTCGTCGAGATACGCGATGAT
GGTAATATGGGATGCACTATC
5'
promoter
RNA
polymerase
Practice Question 4
E)
You also study the expression of different mutants for this gene.
Mutant A has a single base pair substitution with the C/G being replaced with G/C base pair at position
30 (position denoted by the * in the sequence above).
For mutant A answer the following:
Does this mutation change the sequence of the protein produced? Why or why not?
If it does change the sequence of protein be sure to write out the new sequence.
If it does not change the protein sequence, what effect (if any) would you expect it
to have on expression of the gene?
1
20
ORI
40
60
3'...TTCGAGCTCTCGTCGTCGAGATACGCGATGATATTACTGGTAATATGGGGATGCACTATC...5'
5'..AAGCTCGAGAGCAGCAGCTCTATGCGCTACTATAATGACCATTATACCCCTACGTGATAG...3'
promoter
ribosome
Transcribed Image Text:You study the expression of the hexose kinase gene and capture the following electron micrograph of the gene being expressed. mRNA 20 ORI 40 60 3' GATATTACTGGI АAGCTCGAGAGCAGCAGCTCТАTGCGCTACТАТААТGACCАТТАТАСЕССТАCGTGATAG TTCGAGCTCTCGTCGTCGAGATACGCGATGAT GGTAATATGGGATGCACTATC 5' promoter RNA polymerase Practice Question 4 E) You also study the expression of different mutants for this gene. Mutant A has a single base pair substitution with the C/G being replaced with G/C base pair at position 30 (position denoted by the * in the sequence above). For mutant A answer the following: Does this mutation change the sequence of the protein produced? Why or why not? If it does change the sequence of protein be sure to write out the new sequence. If it does not change the protein sequence, what effect (if any) would you expect it to have on expression of the gene? 1 20 ORI 40 60 3'...TTCGAGCTCTCGTCGTCGAGATACGCGATGATATTACTGGTAATATGGGGATGCACTATC...5' 5'..AAGCTCGAGAGCAGCAGCTCTATGCGCTACTATAATGACCATTATACCCCTACGTGATAG...3' promoter ribosome
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