Biology (MindTap Course List)
Biology (MindTap Course List)
11th Edition
ISBN: 9781337392938
Author: Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher: Cengage Learning
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Chapter 14, Problem 13TYU

INTERPRET DATA Develop a simple hypothesis that would explain the behavior of each of the following types of mutants in E. coli.

Mutant a: The map position of this mutation is in the trp operon. The mutant cells are constitutive; that is, they produce all the enzymes coded for by the trp operon, even if large amounts of tryptophan are present in the growth medium. Mutant b: The map position of this mutation is in the trp operon. The mutant cells do not produce any enzymes coded for by the trp operon under any conditions. Mutant c: The map position of this mutation is some distance from the trp operon. The mutant cells are constitutive; that is, they produce all the enzymes coded for by the trp operon, even if the growth medium contains large amounts of tryptophan.

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O Macmillan Learning Glu-His-Trp-Ser-Gly-Leu-Arg-Pro-Gly The pKa values for the peptide's side chains, terminal amino groups, and carboxyl groups are provided in the table. Amino acid Amino pKa Carboxyl pKa Side-chain pKa glutamate 9.60 2.34 histidine 9.17 1.82 4.25 6.00 tryptophan 9.39 2.38 serine 9.15 2.21 glycine 9.60 2.34 leucine 9.60 2.36 arginine 9.04 2.17 12.48 proline 10.96 1.99 Calculate the net charge of the molecule at pH 3. net charge at pH 3: Calculate the net charge of the molecule at pH 8. net charge at pH 8: Calculate the net charge of the molecule at pH 11. net charge at pH 11: Estimate the isoelectric point (pl) for this peptide. pl:
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