The following images represent confocal images of cells with full-length Mira protein (Mira 1-830), which localizes to the plasma membrane, and truncations of the Mira protein that either localize to the plasma membrane and/or the nucleus. How would you determine whether the membrane localization sequence of Mira is sufficient? Mra 130 Mira 1-727 | Mira 1-048 Mra 1-465 Mira 1-329 Mira 1-290 Mra 1-100 Ma 94-290 Remove amino acids 94-290 from Mira and examine the localization of the protein Remove amino acids 1-106 from Mira and examine the localization of the protein Add amino acids 1-830 from Mira to GFP and examine localization of GFP Add amino acids 94-290 from Mira to GFP and examine localization of GFP Add amino acids 1-106 from Mira to GFP and examine localization of GFP
The following images represent confocal images of cells with full-length Mira protein (Mira 1-830), which localizes to the plasma membrane, and truncations of the Mira protein that either localize to the plasma membrane and/or the nucleus. How would you determine whether the membrane localization sequence of Mira is sufficient? Mra 130 Mira 1-727 | Mira 1-048 Mra 1-465 Mira 1-329 Mira 1-290 Mra 1-100 Ma 94-290 Remove amino acids 94-290 from Mira and examine the localization of the protein Remove amino acids 1-106 from Mira and examine the localization of the protein Add amino acids 1-830 from Mira to GFP and examine localization of GFP Add amino acids 94-290 from Mira to GFP and examine localization of GFP Add amino acids 1-106 from Mira to GFP and examine localization of GFP
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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
Transcribed Image Text:**Confocal Imaging of Mira Protein Variants**
The images above showcase confocal microscopy of cells expressing various forms of the Mira protein, ranging from the full-length version (Mira 1-830) to different truncated versions. Mira 1-830 localizes to the plasma membrane, and the study investigates whether truncations localize similarly to the membrane or to the nucleus.
**Image Details:**
- **A: Mira 1-830**: Localization at the plasma membrane (clear outline visible).
- **B: Mira 1-727**: Shows membrane localization.
- **C: Mira 1-644**: Displays localization similar to previous images.
- **D: Mira 1-405**: Appears concentrated differently, possibly more intracellular.
- **E: Mira 1-329**: Similar to Mira 1-405 with noticeable intracellular presence.
- **F: Mira 1-209**: Mainly localized intracellularly, diminishes membrane association.
- **G: Mira 1-106**: Primarily nuclear localization, further reduced membrane presence.
- **H: Mira 94-290**: Displays a distinct pattern, emphasis on different cellular regions.
**Question: Determining Membrane Localization Sequence**
To determine the sufficiency of the membrane localization sequence of Mira, consider the following experimental approaches:
- Remove amino acids 94-290 from Mira and examine protein localization.
- Remove amino acids 1-106 from Mira and examine protein localization.
- Add amino acids 1-830 from Mira to GFP and assess GFP localization.
- Add amino acids 94-290 from Mira to GFP and assess GFP localization.
- Add amino acids 1-106 from Mira to GFP and assess GFP localization.
These methodologies will help identify the essential sequences for membrane localization by observing the changes in localization patterns in the resultant proteins.
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