11. You isolated a gene expressed in differentiated hepatocytes in mice. You then fused various fragments of the gene (shown as dark lines in the following figure) to a lacZ reporter lacking either enhancers or a promoter. The resulting clones were introduced into hepatocytes in tissue culture, and the level of lacZ expression was monitored by using a colorimetric assay. From the results that follow, which region contains a likely hepatocyte enhancer? a. a b. b с. с d. d e. e lacz expression 100 gooonnons 5 100 5 5 Fragments fused to lacz lacz A d e ✓ abc E-DO promon

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
icon
Related questions
icon
Concept explainers
Question

please give thorough explanation for why answer is B plz do not answer question if u don't actually understand thank you

11. You isolated a gene expressed in
differentiated hepatocytes in mice. You then fused
various fragments of the gene (shown as dark
lines in the following figure) to a lacZ reporter
lacking either enhancers or a promoter. The
resulting clones were introduced into hepatocytes
in tissue culture, and the level of lacZ expression
was monitored by using a colorimetric assay.
From the results that follow, which region contains
a likely hepatocyte enhancer?
a. a
a
b b
C
1
d. d
e. e
lacz
expression
100
gooonnons
0
0
5
5
100
5
5
Fragments fused to lacz
lacz
S
d e
✓
abc
promon
Transcribed Image Text:11. You isolated a gene expressed in differentiated hepatocytes in mice. You then fused various fragments of the gene (shown as dark lines in the following figure) to a lacZ reporter lacking either enhancers or a promoter. The resulting clones were introduced into hepatocytes in tissue culture, and the level of lacZ expression was monitored by using a colorimetric assay. From the results that follow, which region contains a likely hepatocyte enhancer? a. a a b b C 1 d. d e. e lacz expression 100 gooonnons 0 0 5 5 100 5 5 Fragments fused to lacz lacz S d e ✓ abc promon
Expert Solution
steps

Step by step

Solved in 2 steps with 1 images

Blurred answer
Knowledge Booster
Musculoskeletal system
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education