To identify transcriptional regulatory elements, reporters containing 100 base pairs of Promoter Region DNA were tested in an expression system, where higher numbers indicate higher expression levels. The black boxes indicate the region mutated in each reporter. Based on these results, if you performed 5’ deletion analysis what expression level would you expect from a reporter containing -60 to +1 of the promoter region?| Mutant number | Promoter Region | Reporter gene expression ||---------------|-----------------|--------------------------|| | -100 | -80 | -60 | -40 | -20 | +1 || 1 | | ■ | ■ | ■ | | | 5 || 2 | | ■ | ■ | ■ | ■ | ■ | 5 || 3 | | | ■ | ■ | | ■ | 10 || 4 | | | | ■ | ■ | ■ | 5 || 5 | | ■ | ■ | ■ | ■ | ■ | 0 |Please select the expected expression level from the options below:- [ ] 0- [ ] 40- [ ] 7.5- [ ] 10- [ ] 5**Explanation of Graph:**Each row represents a mutant with specific regions of the promoter mutated (black boxes). The expression levels are measured for different promoter regions:- Mutant 1, 2, and 5 have low expression levels at 5 or 0.- Mutant 3 has the highest expression level at 10.- Mutant 4 shows a moderate expression level at 5.Mutations at different regions impact gene expression differently, showing the importance of specific promoter areas in regulating transcription.

Tỏ idéntify transcriptional regulatory elements, reporters containing 100 base pairs of Promoter Region DNA were tested in an expression system, where higher numbers indicate higher expression levels. The black boxes indicate the region mutated in each reporter. Based on these results, if you performed 5' deletion analysis what expression level would you expect from a reporter containing -60 to +1 of the promoter region? Mutant Promoter Region Reporter gene expression +1 number -100 -80 -60 -40 -20 10 40 7.5 10 身

Tỏ idéntify transcriptional regulatory elements, reporters containing 100 base pairs of Promoter Region DNA were tested in an expression system, where higher numbers indicate higher expression levels. The black boxes indicate the region mutated in each reporter. Based on these results, if you performed 5' deletion analysis what expression level would you expect from a reporter containing -60 to +1 of the promoter region? Mutant Promoter Region Reporter gene expression +1 number -100 -80 -60 -40 -20 10 40 7.5 10 身

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

Please answer asap
Part A You were sequencing a region of 200 kb in Effrenium voratum (a member of the family Symbiodiniaceae) and you found two genes, an insulator, and two enhancers, as shown below. Gene A Promoter gene A Enhancer I Enhancer I| Insulator Promoter gene b Gene B Q: What would happen if you do some gene editing and you move the insulator between both enhancers? The newly positioned insulator allows enhancer II to activate gene B transcription The newly positioned insulator allows enhancer I to hyperactivate gene B transcription The new positioning of the insulator prevents gene A expression It will be unaltered The newly positioned insulator prevents enhancer II from stimulating the transcription of gene B
Which of the following parts of the eukaryotic promoter are bound by general transcription factors? Group of answer choices A promoter proximal elements B enhancer elements C specific promoter elements D the core promoter E the distal promoter
Why must chromatin be remodeled at a eukaryotic promoter? Histone proteins must be added to the promoter so they can recruit RNA polymerase II Histone proteins are blocking promoter sequences recognized by general transcription factors (GTFs) Space needs to be made for the ribosome to bind to a nascent (i.e. newly transcribed) mRNA Because enhancer regions need to be exposed for general transcription factor binding To allow the spliceosome to remove exons from the mRNA during transcription
The APETALA1:GUS line you were given is a transcriptional fusion. Given that APETALA1 encodes a transcription factor, where might you expect to detect the expression of a translational reporter gene fusion?
In a bacterium, most expression regulation occurs because of the strength of a gene’s promoter. What does this refer to? Make sure you describe how two constitutively expressed genes can have lots/little transcription occurring.
Please review my answer for question 10
CD3 is a signaling protein that is typically found only in the plasma membrane of immune system T lymphocytes. CD3 is composed of several different polypeptides, including a gamma chain, CD3γ. Scientists analyzed the promoter of the CD3γ chain gene for regulatory sequences that might have positive or negative effects on expression of the gene. The scientists cloned fragments of the CD3γ gene that included the first transcribed nucleotides plus up to 789 nucleotides of upstream regulatory sequences into plasmids in which the gene for the firefly enzyme luciferase immediately follows the fragments. The plasmids were then introduced into a line of T lymphocytes (Figure 1), and the cells were allowed to grow for a short while. Because the regulatory sequences of the CD3γ gene immediately precede the luciferase gene in the plasmids, the activity, either positive or negative, of the regulatory sequences affected the amount of luciferase gene expression by the T lymphocytes. Luciferase…