To identify transcriptional regulatory elements, reporters containing 100 base pairs of Promoter Region DNA were tested in an expression system, where higher numbers indicate higher expression levels. The black boxes indicate the region mutated in each reporter. Based on these results, if you performed 5’ deletion analysis what expression level would you expect from a reporter containing -60 to +1 of the promoter region?| Mutant number | Promoter Region | Reporter gene expression ||---------------|-----------------|--------------------------|| | -100 | -80 | -60 | -40 | -20 | +1 || 1 | | ■ | ■ | ■ | | | 5 || 2 | | ■ | ■ | ■ | ■ | ■ | 5 || 3 | | | ■ | ■ | | ■ | 10 || 4 | | | | ■ | ■ | ■ | 5 || 5 | | ■ | ■ | ■ | ■ | ■ | 0 |Please select the expected expression level from the options below:- [ ] 0- [ ] 40- [ ] 7.5- [ ] 10- [ ] 5**Explanation of Graph:**Each row represents a mutant with specific regions of the promoter mutated (black boxes). The expression levels are measured for different promoter regions:- Mutant 1, 2, and 5 have low expression levels at 5 or 0.- Mutant 3 has the highest expression level at 10.- Mutant 4 shows a moderate expression level at 5.Mutations at different regions impact gene expression differently, showing the importance of specific promoter areas in regulating transcription.

Tỏ idéntify transcriptional regulatory elements, reporters containing 100 base pairs of Promoter Region DNA were tested in an expression system, where higher numbers indicate higher expression levels. The black boxes indicate the region mutated in each reporter. Based on these results, if you performed 5' deletion analysis what expression level would you expect from a reporter containing -60 to +1 of the promoter region? Mutant Promoter Region Reporter gene expression +1 number -100 -80 -60 -40 -20 10 40 7.5 10 身

Tỏ idéntify transcriptional regulatory elements, reporters containing 100 base pairs of Promoter Region DNA were tested in an expression system, where higher numbers indicate higher expression levels. The black boxes indicate the region mutated in each reporter. Based on these results, if you performed 5' deletion analysis what expression level would you expect from a reporter containing -60 to +1 of the promoter region? Mutant Promoter Region Reporter gene expression +1 number -100 -80 -60 -40 -20 10 40 7.5 10 身

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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