You are studying how the expression of Gene F is regulated. Initially, you identified three potential regulatory elements in the regulatory region upstream of Gene F's transcription start site. To study these potential regulatory elements, you created three different mutants with a different potential regulatory element mutated in each. The data below shows qPCR data for expression of Gene F in Wild-type (Control) samples, each of the three different mutants (Mutant 1-3), and a negative control (No DNA Control). Were you able to identify a regulatory element that binds a transcriptional activator? If so, explain how you determined that and which mutant it is. Make sure to reference specific data from the image and your knowledge of the molecular biology involved in regulating transcription. The dotted line represents our measurement threshold. Fluorescence N Wild-Type Control Mutant 1 Mutant 2 Mutant 3 No DNA Control 10 10 20 30 Cycle

You are studying how the expression of Gene F is regulated. Initially, you identified three potential regulatory elements in the regulatory region upstream of Gene F's transcription start site. To study these potential regulatory elements, you created three different mutants with a different potential regulatory element mutated in each. The data below shows qPCR data for expression of Gene F in Wild-type (Control) samples, each of the three different mutants (Mutant 1-3), and a negative control (No DNA Control). Were you able to identify a regulatory element that binds a transcriptional activator? If so, explain how you determined that and which mutant it is. Make sure to reference specific data from the image and your knowledge of the molecular biology involved in regulating transcription. The dotted line represents our measurement threshold. Fluorescence N Wild-Type Control Mutant 1 Mutant 2 Mutant 3 No DNA Control 10 10 20 30 Cycle

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter10: From Proteins To Phenotypes

Section: Chapter Questions

Problem 21QP: Transcriptional regulators are proteins that bind to promoters (the 5-flanking regions of genes) to...

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Question

You are studying how the expression of Gene F is regulated. Initially, you identified three potential
regulatory elements in the regulatory region upstream of Gene F's transcription start site. To study
these potential regulatory elements, you created three different mutants with a different potential
regulatory element mutated in each. The data below shows qPCR data for expression of Gene F in
Wild-type (Control) samples, each of the three different mutants (Mutant 1-3), and a negative
control (No DNA Control). Were you able to identify a regulatory element that binds a
transcriptional activator? If so, explain how you determined that and which mutant it is. Make sure
to reference specific data from the image and your knowledge of the molecular biology involved in
regulating transcription. The dotted line represents our measurement threshold.
Fluorescence
N
Wild-Type Control
Mutant 1
Mutant 2
Mutant 3
No DNA Control
10
10
20
30
Cycle

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