The bacterial operon (a set of co-expressed genes) shown below contains one promoter (green), two terminators (stop signs), and three genes (coloured boxes). If 100 units of the red gene product are expressed, how much of each of the blue gene products are made? 오 00 100 200 It depends

The bacterial operon (a set of co-expressed genes) shown below contains one promoter (green), two terminators (stop signs), and three genes (coloured boxes). If 100 units of the red gene product are expressed, how much of each of the blue gene products are made? 오 00 100 200 It depends

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

An activator (protein “V” – not encoded by this operon) binds to the activating region and RNA polymerase is bound to promoter. Describe what is occurring in terms of operon regulation/control.
Based on the partial diploid sketched shown in Figure 3, where cross-hatched rectangles and the red bar indicate mutations that inactivate that particular genetic element of the operon, state if expression of LacO (Lac Operator) and LacY (Lac Permease) is (select one of the options below for each genetic element and briefly justify your answer): • constitutively ON (in the presence/absence of lactose) • constitutively OFF (in the presence/absence of lactose) • inducible by lactose • not enough information given to conclude • none of the above FIGURE 3: lact laco lacz- lacř lact laco lacz lac
Given the Table below concerning the lac operon, match the letter with what would be occurring for each scenario. Repressor is bound to the operator, cAMP complex is bound and transcription is prevented. Allolactose is bound to the repressor, cAMP complex is bound, and transcription is prevalent. Allolactose is bound to the repressor, cAMP complex is not assembled, and transcription is slow Repressor is bound to the operator, cAMP complex is not assembled, and transcription is prevented.
Give the levels of Beta-galactosidase activity (high or low or zero) expected for the following partial diploids for the lac operon strains.
What would be the transcriptional output of the lac operon in an E. coli cell in the following 8 scenarios? Your answers must be one word each, selected from the following two options: 'high' and 'low.
Examine the following theoretical operon (gen) in E. coli which contains structural genes (genC, genB, genA) that are involved in amino acid biosynthesis. The gen operon also includes the gene encoding for the regulator molecule within it. When the amino acid that this operon helps synthesize is present, it binds to the regulator forming a complex which can bind to the operator region. Assume that the regulator molecule only binds to the operator in the presence of the amino acid. Promoter || Operator genC genB gen operon Regulator genA
The fictional bacteria Plumbumbacter aurus converts the lead ions to gold ions and uses the resulting electrons to create ATP. aurus has the midas operon which includes the promoter (midasP), the operator (midasO), and eight genes that encode proteins involved in converting lead to gold. The midas operon is regulated by the protein encoded by the midasR gene and the presence of gold ions. When the concentration of gold ions is low, the midas operon is transcribed and the bacteria convert lead into gold. When the concentration of gold is high, the midas operon is off. To better understand regulation of the midas operon, you created P. aurus mutants. The midasR- mutant has a nonfunctional midasR, the midasO- mutant has a nonfunctional midasO, and the midasP-mutant has a nonfunctional midasP. Based on the data below, the midas operon has what type of transcription control? (negative inducible, negative repressible, positive inducible, positive repressible) Strain Produces Gold in…
The lac operon produces: (choose the best answer) OA single mRNA molecule O A single protein OA single mRNA molecule and a single protein O Multiple proteins OA single mRNA molecule and multiple proteins
Decide which operon each of the following characteristics applies to. Note: a description may apply to both. a. lac operon b. trp operon 68. repressible 69. Turned off when end product is present (feedback inhibition) 70. Promoter, activator region, and 3 genes 71. Repressible 72. contain operator and promoter regions as well as transcript units of at least two or more coding genes 73. Codes for catabolic enzymes that break down lactose 74. Inducible 75. Promoter, activator region, and 5 genes 76. Codes for anabolic enzymes that help manufacture tryptophan 77. Lactose is the co-inducer 78. Turned on when substrate is present 79. Tryptophan is the co-repressor 80. Regulatory gene segments
The following table lists 4 bacterial strains that are partial diploids for lac operon genes. Given the activity of beta-galactosidase measured for each strain in the absence (-lac) or presence (+lac) of lactose, complete the table by choosing the appropriate symbol (+, -, C, S) to indicate the allele of the gene or site missing from the table (blue numbers). strain A BC 5 C D 7 chromosome I O 1 2 4 1 [Select] 9 3 [Select] [Select] [Select] 9 [Select] + + Z + + 6 + I +5 + 10 plasmid O 3 + 7 C Z + 8 8 2 [Select] 4 [Select] 6 [Select] B-gal act. -lac +lac 0.002 0.003 0.002 0.058 0.063 0.121 0.059 0.062 Select] 1 ✔ [ Select] + is C S
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