You are studying the ABCD operon from a newly isolated bacterium. Which of the following would result in expression of proteins A, B, and C but not D? C promoter Mutation in D that causes degradation of the mRNA Mutation in A that removes the translational start site All of these options Mutation of sequences in the promoter needed for RNA Polymerase to bind Insertion of a transcriptional termination site in the beginning of the D sequence

You are studying the ABCD operon from a newly isolated bacterium. Which of the following would result in expression of proteins A, B, and C but not D? C promoter Mutation in D that causes degradation of the mRNA Mutation in A that removes the translational start site All of these options Mutation of sequences in the promoter needed for RNA Polymerase to bind Insertion of a transcriptional termination site in the beginning of the D sequence

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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The fictional bacteria Plumbumbacter aurus converts the lead ions to gold ions and uses the resulting electrons to create ATP. aurus has the midas operon which includes the promoter (midasP), the operator (midasO), and eight genes that encode proteins involved in converting lead to gold. The midas operon is regulated by the protein encoded by the midasR gene and the presence of gold ions. When the concentration of gold ions is low, the midas operon is transcribed and the bacteria convert lead into gold. When the concentration of gold is high, the midas operon is off. To better understand regulation of the midas operon, you created P. aurus mutants. The midasR- mutant has a nonfunctional midasR, the midasO- mutant has a nonfunctional midasO, and the midasP-mutant has a nonfunctional midasP. Based on the data below, the midas operon has what type of transcription control? (negative inducible, negative repressible, positive inducible, positive repressible) Strain Produces Gold in…
What is a specific region in the trp operon that you could mutate so it expresses the trp operon in conditions of excess tryptophan. Why will this mutation cause this? Operon structure: promoter, operater, structural genes promoter - DNA region that is the site of RNA polymerase sigma binding and transcription initiation operator – DNA region that influences gene expression through its interaction with a protein (transcription factor) structural genes – gene whose expression is being regulated; encodes a polypeptide or functional RNA
The operator of the lactose operon in E. Coli is located: between the promoter and the repressor gene upstream of the promoter between the promoter and the structural genes anywhere on the chromosome among the structural genes
The organization and function of the lac operon in E. coli is shown in the following figures: XXI Transcription Repressor mRNA Translation RNA polymerase M M Flask ZYA XXXX Active M-repressor protein Repressor active, operon off. The repressor protein binds with the operator, preventing transcription from the operon. (a) An inducible operon Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings Allolactose (inducer) Carbon/Energy Source 0 Z MInactive repressor protein (a) An inducible operon Transcription Translation XXX Permease B-Galactosidase Transacetylase Operon mRNA 3 Repressor inactive, operon on. When the inducer allolactose binds to the repressor protein, the inactivated repressor can no longer block transcription. The structural genes are transcribed, ultimately resulting in the production of the enzymes needed for lactose catabolism. Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings Suppose you inoculate three flasks of minimal…
Decide which operon each of the following characteristics applies to. Note: a description may apply to both. a. lac operon b. trp operon 68. repressible 69. Turned off when end product is present (feedback inhibition) 70. Promoter, activator region, and 3 genes 71. Repressible 72. contain operator and promoter regions as well as transcript units of at least two or more coding genes 73. Codes for catabolic enzymes that break down lactose 74. Inducible 75. Promoter, activator region, and 5 genes 76. Codes for anabolic enzymes that help manufacture tryptophan 77. Lactose is the co-inducer 78. Turned on when substrate is present 79. Tryptophan is the co-repressor 80. Regulatory gene segments
When referring to attenuation in the regulation of the trp operon it would be safe to say that, when there are high levels of tryptophan available to the organism, transcription termination at the attenuator region is likely the ribosomes stall during translation of the attenuator region translation termination of the trp operon is likely the trp operon is transcribed at relatively high levels tryptophan inactivates the repressor protein
You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation a.Which operons would be functional under these conditions? b.What repressors would be made? (NOTE: name the repressors using their gene names) c.Which repressor(s) would be made in the inactive form? d.Which repressor(s) would be made in the active form? e.Which repressor(s) under these conditions can bind the operator sequence? f.Which repressor(s)under these conditions cannot bind the operator sequence?
A bacterial species has a hypothetical sigma promoter that has the following sequence: TTGGCA - 18 bases - TATAAT What change in the level of transcription would there be if the sequence was mutated to: TTCGCA -18 bases -TATAAT O The mutation would bind the promoter to the consensus and produce normal levels of transcription O The mutation would inhibit the promoter thereby inhibiting transcription The mutation would move the promoter away from consensus and reduce the level of transcription O No change the consensus TATAAT sequence in the same. D00 F3 F4 F5 F6 F7 F8 F9 % & 5 6 7 8 9 %24
Which of the following statements is false? a mutation in a 5' or 3' splice site must alter the sequence of the protein encoded by a gene a missense mutations replaces one amino acid with a different amino acid a mutation in a promoter is unlikely to alter the sequence of the polypeptide encoded by a gene a mutation in a transcriptional terminator is unlikely to alter the sequence of a protein encoded by a gene. a frameshift mutation changes the sequence of a protein 0000
A mutation at the operator prevents the regulator protein from binding.What effect will this mutation have in the following types of operons? Q. Regulator protein is a repressor of a repressible operon.
There is a mutation in sequence of the lac operator that prevents the lac repressor from being able to recognize and bind to it. What would the status of transcription of the lac operon be under each of the following circumstances? Explain your answers. a-Lactose present, glucose present b-Lactose present, glucose absent c-Lactose absent, glucose present d-Lactose absent, glucose absent
The promoter of an operon is the site to which RNA polymerase binds to begin transcription. Certain base changes in the promoter result in a mutant site that RNA polymerase can’t bind. Would you expect mutations in the promoter that prevents binding of RNA to act in trans on another copy of the operon on a plasmid or only in cis on the copy immediately adjacent to the mutated site?