The promoter of an operon is the site to which RNA polymerase binds to begin transcription. Certain base changes in the promoter result in a mutant site that RNA polymerase can’t bind. Would you expect mutations in the promoter that prevents binding of RNA to act in trans on another copy of the operon on a plasmid or only in cis on the copy immediately adjacent to the mutated site?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
The promoter of an operon is the site to which RNA polymerase binds to begin transcription. Certain base changes in the promoter result in a mutant site that RNA polymerase can’t bind. Would you expect mutations in the promoter that prevents binding of RNA to act in trans on another copy of the operon on a plasmid or only in cis on the copy immediately adjacent to the mutated site?
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