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Suppose you inoculate three flasks of minimal medium with E. coli. The carbon/energy source in each case is shown in the following table: Flask A B C Carbon/Energy Source glucose glucose and lactose lactose Assume that the glucose does not act as an ON/OFF switch, but rather as a regulator of the rate of transcription (like a dimmer switch on a light). A. After a few hours of incubation, which of these flasks WILL NOT have the enzyme beta-galactosidase present in the cells? Explain your answer. B. Suppose that in Flask C you have cells that are mutated such that their lactose operon is missing the region called P in the diagrams above. Will these cells contain beta-galactosidase? Explain your answer. C. Suppose that in Flask A you have cells that are mutated such that the operon is missing the region called O in the diagrams above. Will these cells contain beta-galactosidase? Explain your answer.

Suppose you inoculate three flasks of minimal medium with E. coli. The carbon/energy source in each case is shown in the following table: Flask A B C Carbon/Energy Source glucose glucose and lactose lactose Assume that the glucose does not act as an ON/OFF switch, but rather as a regulator of the rate of transcription (like a dimmer switch on a light). A. After a few hours of incubation, which of these flasks WILL NOT have the enzyme beta-galactosidase present in the cells? Explain your answer. B. Suppose that in Flask C you have cells that are mutated such that their lactose operon is missing the region called P in the diagrams above. Will these cells contain beta-galactosidase? Explain your answer. C. Suppose that in Flask A you have cells that are mutated such that the operon is missing the region called O in the diagrams above. Will these cells contain beta-galactosidase? Explain your answer.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Suppose you inoculate three flasks of minimal medium with E. coli. The carbon/energy source in each case is shown in the following table: Flask A B C Carbon/Energy Source glucose glucose and lactose lactose Assume that the glucose does not act as an ON/OFF switch, but rather as a regulator of the rate of transcription (like a dimmer switch on a light). A. After a few hours of incubation, which of these flasks WILL NOT have the enzyme beta-galactosidase present in the cells? Explain your answer. B. Suppose that in Flask C you have cells that are mutated such that their lactose operon is missing the region called P in the diagrams above. Will these cells contain beta-galactosidase? Explain your answer. C. Suppose that in Flask A you have cells that are mutated such that the operon is missing the region called O in the diagrams above. Will these cells contain beta-galactosidase? Explain your answer.
Transcribed Image Text:Suppose you inoculate three flasks of minimal medium with E. coli. The carbon/energy source in each case is shown in the following table: Flask A B C Carbon/Energy Source glucose glucose and lactose lactose Assume that the glucose does not act as an ON/OFF switch, but rather as a regulator of the rate of transcription (like a dimmer switch on a light). A. After a few hours of incubation, which of these flasks WILL NOT have the enzyme beta-galactosidase present in the cells? Explain your answer. B. Suppose that in Flask C you have cells that are mutated such that their lactose operon is missing the region called P in the diagrams above. Will these cells contain beta-galactosidase? Explain your answer. C. Suppose that in Flask A you have cells that are mutated such that the operon is missing the region called O in the diagrams above. Will these cells contain beta-galactosidase? Explain your answer.
The organization and function of the lac operon in E. coli is shown in the following figures: XXI Transcription Repressor mRNA Translation RNA polymerase M M Flask ZYA XXXX Active M-repressor protein Repressor active, operon off. The repressor protein binds with the operator, preventing transcription from the operon. (a) An inducible operon Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings Allolactose (inducer) Carbon/Energy Source 0 Z MInactive repressor protein (a) An inducible operon Transcription Translation XXX Permease B-Galactosidase Transacetylase Operon mRNA 3 Repressor inactive, operon on. When the inducer allolactose binds to the repressor protein, the inactivated repressor can no longer block transcription. The structural genes are transcribed, ultimately resulting in the production of the enzymes needed for lactose catabolism. Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings Suppose you inoculate three flasks of minimal medium with E. coli. The carbon/energy source in each case is shown in the following table:
Transcribed Image Text:The organization and function of the lac operon in E. coli is shown in the following figures: XXI Transcription Repressor mRNA Translation RNA polymerase M M Flask ZYA XXXX Active M-repressor protein Repressor active, operon off. The repressor protein binds with the operator, preventing transcription from the operon. (a) An inducible operon Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings Allolactose (inducer) Carbon/Energy Source 0 Z MInactive repressor protein (a) An inducible operon Transcription Translation XXX Permease B-Galactosidase Transacetylase Operon mRNA 3 Repressor inactive, operon on. When the inducer allolactose binds to the repressor protein, the inactivated repressor can no longer block transcription. The structural genes are transcribed, ultimately resulting in the production of the enzymes needed for lactose catabolism. Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings Suppose you inoculate three flasks of minimal medium with E. coli. The carbon/energy source in each case is shown in the following table:
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