Numerous diseases are treated by injection of polypeptide growth factors, such as human growth factor. A project team in a pharmaceutical company wishes to produce high levels of a growth factor for therapeutic use by overexpressing and purifying the protein from E coli. The team takes the cDNA of the human gene and makes the mutations below before transferring the gene to E coli for high level protein expression. As seen in the gel below, the mutations dramatically enhanced the levels of protein produced (growth factor indicated by arrows). Explain why the team altered the human cDNA of the growth factor as shown below for expression in E coli (limit 3-4 sentences)
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Numerous diseases are treated by injection of polypeptide growth factors, such as human growth factor. A project team in a pharmaceutical company wishes to produce high levels of a growth factor for therapeutic use by overexpressing and purifying the protein from E coli. The team takes the cDNA of the human gene and makes the mutations below before transferring the gene to E coli for high level protein expression. As seen in the gel below, the mutations dramatically enhanced the levels of protein produced (growth factor indicated by arrows). Explain why the team altered the human cDNA of the growth factor as shown below for expression in E coli (limit 3-4 sentences).
Step by step
Solved in 3 steps
