Consider a mutant of E. coli that has an inactivating mutation in the gene for the catabolite activator protein (cap). Choose the compound(s) that could be provided to the mutant to achieve full transcription of the lac operon. If no combination of compounds can allow for transcription, chose none. O ATP O CAMP Oglucose lactose none

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**Educational Resource: Transcription in E. coli Lac Operon**

**Consider a mutant of *E. coli* that has an inactivating mutation in the gene for the catabolite activator protein (*cap⁻*). Choose the compound(s) that could be provided to the mutant to achieve full transcription of the lac operon. If no combination of compounds can allow for transcription, choose none.**

- ☐ ATP
- ☐ cAMP
- ☐ glucose
- ☐ lactose
- ☐ none

**Explanation:**

This question deals with the regulatory mechanisms of the lac operon in *E. coli*. The lac operon includes genes that are involved in the metabolism of lactose. The regulation of this operon involves several key molecules and proteins, and one of them is the catabolite activator protein (CAP), which requires cyclic AMP (cAMP) to function properly. A mutation in the gene for CAP (CAP⁻) disrupts the normal regulation and can prevent the transcription of the lac operon.

- **ATP**: Adenosine triphosphate is the energy currency of the cell but does not directly influence the transcription of the lac operon.
- **cAMP**: Cyclic AMP is required for CAP to bind to the promoter region of the lac operon to facilitate transcription.
- **glucose**: The presence of glucose inhibits the production of cAMP, thus preventing CAP from activating transcription.
- **lactose**: The presence of lactose serves as the inducer for the lac operon, enabling the transcription of the necessary genes when glucose is not present.
- **none**: This option is chosen if no combination of the compounds can restore transcription of the lac operon.

For the transcription of the lac operon in a CAP⁻ mutant, it is essential to consider how each compound affects the overall regulatory process.
Transcribed Image Text:**Educational Resource: Transcription in E. coli Lac Operon** **Consider a mutant of *E. coli* that has an inactivating mutation in the gene for the catabolite activator protein (*cap⁻*). Choose the compound(s) that could be provided to the mutant to achieve full transcription of the lac operon. If no combination of compounds can allow for transcription, choose none.** - ☐ ATP - ☐ cAMP - ☐ glucose - ☐ lactose - ☐ none **Explanation:** This question deals with the regulatory mechanisms of the lac operon in *E. coli*. The lac operon includes genes that are involved in the metabolism of lactose. The regulation of this operon involves several key molecules and proteins, and one of them is the catabolite activator protein (CAP), which requires cyclic AMP (cAMP) to function properly. A mutation in the gene for CAP (CAP⁻) disrupts the normal regulation and can prevent the transcription of the lac operon. - **ATP**: Adenosine triphosphate is the energy currency of the cell but does not directly influence the transcription of the lac operon. - **cAMP**: Cyclic AMP is required for CAP to bind to the promoter region of the lac operon to facilitate transcription. - **glucose**: The presence of glucose inhibits the production of cAMP, thus preventing CAP from activating transcription. - **lactose**: The presence of lactose serves as the inducer for the lac operon, enabling the transcription of the necessary genes when glucose is not present. - **none**: This option is chosen if no combination of the compounds can restore transcription of the lac operon. For the transcription of the lac operon in a CAP⁻ mutant, it is essential to consider how each compound affects the overall regulatory process.
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