Amount of B-Galactosidase (% of mutant strain Addition of Lactose in the presence of lactose) Mutant No 100 Mutant Yes 100 Merozygote No 100 Merozygote Yes 200 Explain the nature of the defect in the mutant strain.
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
Suppose that you have isolated a mutant strain of E. coli in
which the lac operon is constitutively expressed. To understand
the nature of this defect, you create a merozygote in which the
mutant strain contains an F′ factor with a normal lac operon
and a normal lacI gene. You then compare the mutant strain and
the merozygote with regard to their β-galactosidase activities in
the presence and absence of lactose. You obtain the following
results:
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