a. The original constitutive operator mutations in thelac operon were all base changes in o1. Why doyou think mutations in o2 or o3 were not isolated inthese screens?b. Explain how a mutagen that causes small insertions could produce an ocmutation.c. Would a strain with one of the ocmutations described in part (b) and also a lacIS mutation be ableto make β-galactosidase either in the presence orabsence of inducer? Explain
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a. The original constitutive operator mutations in the
lac operon were all base changes in o1. Why do
you think mutations in o2 or o3 were not isolated in
these screens?
b. Explain how a mutagen that causes small insertions could produce an oc
mutation.
c. Would a strain with one of the oc
mutations described in part (b) and also a lacIS mutation be able
to make β-galactosidase either in the presence or
absence of inducer? Explain
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