. One mechanism by which antisense RNAs act as negative regulators of gene expression is by base pairingwith the ribosome binding site on the sense mRNA toblock translation. In a second, alternative mechanism,the act of transcribing an antisense RNA can somehow prevent RNA polymerase from recognizing thesense promoter for the same gene. Design an experimental approach that would enable you to distinguishbetween these two modes of action at a specific gene.(Hint: What would be the outcome in each case ifhigh levels of the antisense RNA were transcribedfrom a gene on a plasmid?)
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
. One mechanism by which antisense RNAs act as negative regulators of gene expression is by base pairing
with the ribosome binding site on the sense mRNA to
block translation. In a second, alternative mechanism,
the act of transcribing an antisense RNA can somehow prevent RNA polymerase from recognizing the
sense promoter for the same gene. Design an experimental approach that would enable you to distinguish
between these two modes of action at a specific gene.
(Hint: What would be the outcome in each case if
high levels of the antisense RNA were transcribed
from a gene on a plasmid?)
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
