The trp repressor binds to the operator region of DNA as a dimer (two repressor protein subunits bound together as a unit). You isolate a new mutant e, coli strain that you call Rnovel. In this strain, the repressor protein cannot attach to DNA (it cannot bind to the operator) but it can make dimers. In this strain, how is expression of the trp operon regulated? A. inducible OB. noninducible OC. constitutive OD. repressible

Q: When researchers analyzed the gad operon they found the following: Expression of the gad operon at…

A: Here we saw that mutant 1 is causing the expression of gad protein even at pH7 which was not…

Q: please answer Why is it useful to have two different mechanisms of positive regulation? I.e. why…

A: The L-arabinose operon, also called the ara or araBAD operon, is an operon required for the…

Q: In the lac operon system, do the inducers act at the transcription or translation level? Explain why…

A: The lactose operon (lac operon) consists of a regulatory gene (i-gene) and three structural genes -…

Q: 1) The logic of the Lac operon was deciphered by using bacterial lac-operon mutants. Crucial to the…

A: Lac operon is a genetic unit in bacteria that helps in the metabolism of lactose in E.coli and lac…

Q: If an abnormal repressor protein could still bind allolactose but the binding of allolactose did not…

A: The Lac operon contains genes responsible for the transportation and breakdown of lactose. These…

Q: If an abnormal repressor protein could still bind allolactose but the binding of allolactose did not…

A: Operon acts as a single regulated unit having one or more structural genes, promoter gene, a…

Q: Strain X-23 has a mutation in the lacI gene resulting in a lacI protein that cannot bind DNA.…

A: Gene regulation at the level of transcription in bacteria is achieved by the operon model. Operon…

Q: The TRP operon functions by attenuation. Briefly explain how attenuation works and why it is…

A: Trp operon or the tryptophan operon is a group of genes that are responsible for tryptophan enzymes.…

Q: A mutation in the operator region of the trp operon can prevent the trp repressor from binding to…

A: Operons are the functional unit of transcription and regulation of genes in almost all bacteria and…

Q: In the lac operon (below), how will expression of the genes lacZ and lacy be effect by a mutation in…

A: Lac operon includes the genes that code for the enzymes involved in the catabolism of lactose sugar.…

Q: If you add a co-eepressor to a repressor protein and that allows it to bind the operator is this a…

A: Inducible operon is one that is expressed in the presence of inducer. Constitutive operon is the…

Q: A mutation at the operator prevents the regulator protein from binding. What effect will this…

A: An operon includes a group of genes that have a common promoter, regulator, and would be transcribes…

Q: If lactose isn't present in the environment of an E. coli, what is the state of the lac operon? O a.…

A: The lactose operon (also known as the lac operon) is a group of genes present in E. coli and other…

Q: Propose a final method of gene regulation of the Up Lateoperon using an updated drawn figure of the…

A: An operon is a part of the DNA where a few genes are clustered together that are transcribed…

Q: Which of the following statements is not true of both the LacI and TrpR repressor proteins?a) an…

A: The objective of the question is to identify the statement that is not true for both LacI and TrpR…

Q: a. The original constitutive operator mutations in thelac operon were all base changes in o1. Why…

A: The original constitutive operator mutations made in the lac operon involved base changes in the O1.…

Q: The trp operon in E. coli encodes enzymes essential for the biosynthesis of tryptophan. In the…

A: trp operon is a repressor operon found in E. coli bacteria, which consists of a group of genes that…

Q: Strain X-23 has a mutation in the lacI gene resulting in a lacI protein that cannot bind DNA.…

A: The operon model is applicable in bacterial gene regulation and some lower eukaryotes gene…

Q: Is the regulator protein that binds to the operator of this operon a repressor (negative control) or…

A: Lac operon is a concept of gene expression and its control system by oppressor, repressor, and…

Q: You identify a mutant that affects lac operon expression. You decide to investigate this mutant…

A: The concept of transcriptional regulation refers to the mechanisms by which cells control the…

Q: . Partial diploid forms of E. coli were created, each of which contained a com- plete lactose operon…

A: Lac operon is composed of lacI, P, lacO, lacZ, lacY and lacA genes. Regulatory genes are lacI, P and…

Q: On its chromosome, an E. coli cell has a genotype of lacI− lacZ+ lacY+ lacA+. It has an F′ factor…

A: Lac operon is studied extensively by using mutant strains of E.coli. It is studied under the domain…

Q: Attardi et al (1963) found out that lactose operon mRNA synthesis in E. coli increased with the…

A: Introduction Operon is a genetic regulatory system found in bacteria and their viruses that clusters…

Q: A mutation in the repressor LacI causes it to lose the ability to bind its effector, allolactose.…

A: The question is asking about the effect of a mutation in the LacI repressor that causes it to lose…

Q: In the bacterium,Martian coli, it was discovered that the lac operon is positively regulated.…

A: Operon is a functional unit of DNA that consists of cluster of genes under control of promoter.…

Q: If an abnormal repressor protein could still bind allolactose, but thebinding of allolactose did not…

A: Deoxyribonucleic acid is a particle made out of two polynucleotide chains that loop around one…

Q: A bacterial cell has very low glucose and lactose is present.  Explain how the lac operon will…

A: Lac Operon -- An operon is a functioning unit of genomic DNA which contains a group of genes…

Q: If a wild-type (normal, NOTmutated) E. coli strain is grown in a medium: a. without lactose or…

A: The lac operon or the lactose operon is a mechanism that is involved in the metabolism and transport…

Q: You have isolated a series of mutants affecting regulation of the lac operon. All of these are…

A: The lac (lactose) operon works in the prokaryotic cells and regulates the metabolism of the lactose.…

Q: In E. coli, when glucose is present, galactose is used to make a component of the bacterial cell…

A: In the absence of glucose the operon is not described or transcribed at very low levels. When…

Q: If CAP is bound to the Promoter of the Lac Operon and the repressor protein is not bound to the…

A: Operon is the prokaryotic gene regulatory system that controls the expression of polycistronic mRNA.…

Q: You identify a new transcriptional regulator that binds a known sequence of DNA. You are able to…

A: Identify a new transcriptional regulator that binds a known sequence of DNA.Observe the behavior of…

Q: For the following types of transcriptional control, indicate whether the protein produced by the…

A: An inducible operon is one which is not transcribed. For transcription of the molecule, it requires…

Q: Strain LOLZ has a mutation in the lacI gene that results in a lacI protein that cannot bind…

A: Lac operon is the segment of DNA which is responsible for breaking down lactose into glucose and…

Q: that

A: The trp operon found in E coli bacteria . It's a group of genes that encode biosynthetic enzymes.…

Q: Which of the following describes the lac operon in E. coli when lactose and glucose is present in…

A: The lac repressor acts as a lactose sensor. It normally blocks transcription of the operon, but…

Q: Which statement about the transcription attenuation mechanism is TRUE? 1.In some operons (e.g., the…

A: Transcriptional attenuation is a regulatory mechanism that causes premature termination of…

Q: he lac operon, determine whether genes will be expressed (on) or silenced (off) given the following:…

A: IN option A Lactose levels are high, glucose is low.---- Genes will be expressed(on).

Q: You have created a null mutation of tRNA synthase for trp-tRNA (the synthase enzyme attaches the trp…

A: A null mutation is a mutation that completely removes a gene or part of a gene.In this case, the…

Q: Tryptophan (Trp) attenuation is an extra mechanism of regulation of trp operon when trp level is too…

A: Attenuation is a mechanism of control of the tryptophan (Trp) operon that occurs when the level of…

Q: ou have isolated different mutants (reg1 and reg2) causing constitutive expression of the emu operon…

A: The operon's regulation can take place positively or negatively. If the negative operator is bound…

Q: Examine the following theoretical operon (gen) in E. coli which contains structural genes (genC,…

A: Question 1: B. The amino acid is bound to the regulator, causing the regulator to bind to the…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.