You identify a mutant that affects lac operon expression. You decide to investigate this mutant further by adding a construct into the mutant's cells that contains a wild type version of the full lac operon as well as lacl. When haploid, the lac operon is constitutive. When the wild type construct is added to make a merodiploid, the lac operon is still constitutive. Based on the mutant phenotypes we learned about in class, indicate which of the following might be mutated in the mutant (if there is more than one possibility, then list them all).
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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