You identify a mutant that affects lac operon expression. You decide to investigate this mutant further by adding a construct into the mutant's cells that contains a wild type version of the full lac operon as well as lacl. When haploid, the lac operon is uninducible. When the wild type construct is added to make a merodiploid, the lac operon is still uninducible. Based on the mutant phenotypes we learned about in class, indicate which of the following might be mutated in the mutant (if there is more than one possibility, then list them all). The lac repressor tetramerization/dimerization domain is mutated. The lac repressor ligand-binding domain is mutated. The operator is mutated. The lac repressor DNA-binding domain is mutated. O The lac repressor is deleted in the mutant.

Q: 1) The logic of the Lac operon was deciphered by using bacterial lac-operon mutants. Crucial to the…

A: Lac operon is a genetic unit in bacteria that helps in the metabolism of lactose in E.coli and lac…

Q: If an abnormal repressor protein could still bind allolactose but the binding of allolactose did not…

A: The Lac operon contains genes responsible for the transportation and breakdown of lactose. These…

Q: If an abnormal repressor protein could still bind allolactose but the binding of allolactose did not…

A: Operon acts as a single regulated unit having one or more structural genes, promoter gene, a…

Q: Strain X-23 has a mutation in the lacI gene resulting in a lacI protein that cannot bind DNA.…

A: Gene regulation at the level of transcription in bacteria is achieved by the operon model. Operon…

Q: Here is a another cell with 2 copies of the lac operon. Same directions and points as the previous…

A: Lac operon is responsible for break down of lactose into glucose and galactose. It is the segment of…

Q: Here is a cell with copies of lac operon assuming there is no glucose around figure out if the cell…

A: The lactose operon or lac operon is an inducible operon system under dual – positive and negative…

Q: The only way that information is extracted out of DNA is through the transcription of MRNAS that…

A: According to our guideline we can answer only the first question. So upload the second question…

Q: Here is a cell with 2 copies of the lac operon. (assuming there is NO glucose around), figure out if…

A: Lac operon is the segment of DNA which is responsible for controlling the break down of lactose into…

Q: Propose a final method of gene regulation of the Up Lateoperon using an updated drawn figure of the…

A: An operon is a part of the DNA where a few genes are clustered together that are transcribed…

Q: The trp operon in E. coli is a negative repressible operon. This implies that...   a. The trp…

A: The correct answer is:c. The trp operon makes use of a repressor and can be turned "on" (i.e., is…

Q: Operon X produces three enzymes that convert Compound A to Compound B. The R gene makes the…

A: Gene expression is given on the basis of RNA. It is a process through which there is turning on of…

Q: Strain LOLZ has a mutation in the lacI gene that results in a lacI protein that cannot bind…

A: Lac operon is the segment of DNA which is responsible for breaking down of lactose into glucose and…

Q: Consider the lac operon of E. coli. Specifically, explain the following: a. Describe the overall…

A: The lac operon is a collection of genes located in the chromosome of Escherichia coli, and it…

Q: You identify a mutant that affects lac operon expression. You decide to investigate this mutant…

A: The concept of transcriptional regulation refers to the mechanisms by which cells control the…

Q: Explain how the lac operon is regulated when both glucose and lactose is present. Make sure you…

A: An operon is a unit of gene expression that is present in bacteria and it helps to regulate gene…

Q: 5f. Complete the diagrams below to reflect the proteins and any relevant cofactors (e.g. -…

A: The lac operon is an operon, or cluster of genes with one promoter (transcribed as one mRNA). The…

Q: If a wild-type (normal, NOTmutated) E. coli strain is grown in a medium: a. without lactose or…

A: The lac operon or the lactose operon is a mechanism that is involved in the metabolism and transport…

Q: Strain LOLZ has a mutation in the lacI gene that results in a lacI protein that cannot bind…

A: Lac operon is the segment of DNA which is responsible for breaking down lactose into glucose and…

Q: The lactose operon in E. coli comprises two structural genes and three regulatory components.…

A: β-galactosidase activity Permease activity Genotypes Glycerol Lactose Glycerol Lactose A - + -…

Q: Like the lac operon, the hexose operon is controlled by a separate regulatory protein under the…

A: Hexose operon is the set of structural genes, regulatory genes, promoter and operator sequence in…

Q: v constructed gene. What will

A: introduction pGLO is a genetically engineered plasmid which incorporates parts of the arabinose…

Q: For the trp operon, determine whether genes will be expressed (on) or silenced (off) given the…

A: Gene is a basic unit of hereditary information or data that fills a fixed position on a chromosome.…

Q: ac operon.  Drag the listed molecules to their corresponding bin. Reset Help   ONPG IPTG X-Gal…

A: ONPG - beta-galactosidase cleaves this molecule to form a visible yellow product. Explaination:  The…

Q: Given the following mutant and conditions, predict the expression of the lacZ gene (no expression,…

A: Introduction An operon is a functional unit of DNA (Deoxyribonucleic acid) containing a cluster of…

Q: If a wild-type (normal, NOTmutated) E. coli strain is grown in a medium: a. without lactose or…

A: The lac operon or the lactose operon is a mechanism that is involved in the metabolism and transport…

Q: In a bacterial cell with the genotype I-: P+: O+: Z+: Y+: A+, which of the following expression…

A: The lac operon in bacterial cells plays a pivotal role in the regulation of lactose metabolism.…

Q: For the wildtype E. coli two sugars influence the level of transcription of the lactose operon. Fill…

A: Sugars in the growth media cAMP levels (High or Low) Positive regulation occurs (Yes…

Q: Given the Table below concerning the lac operon, match the letter with what would be occurring for…

A: The lac operon is a genetic system found in bacteria, such as E. coli, that regulates the expression…

Q: 9. Give the levels of Beta-galactosidase activity (high or low or zero) expected for the following…

A: A set of genes present in E.coli. that regulates the uptake of lactose from the surrounding…

Q: You have an E.coli strain in which the lac operon is not expressed when glucose is absent and lacto…

A: The lactose operon (also known as the lac operon) is a group of genes present in E. coli and other…

Q: You are growing E. coli in a laboratory in order to study their operons. The growth media you are…

A: The prokaryotic gene regulation is known as operon system in which the expression of polycystronic…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.