To characterize the promoter of the gadA operon you made a series of deletion mutants removing pieces of the promoter to see what would happen. The results are found below: gad promoter gadA gadz -450 xpe3 +1 lacZ activity transcription start site pH 2.0 pH 7.0 A gad promoter beta galactosidase (lac2) +++ 450 gad promoter beta galactosidase (lacz) +++ +++ 300 gad promoter beta galactosidase (lacZ) 150 D gad promoter beta galactosidase (lacz) -450 -150 gad promoter beta-galactosidase (lacZ) -450 -300 Based on these results, what can you conclude about the gad promoter? O a. The promoter is only regulated by repression Ob. The promoter is regulated by a mix of activation and repression O c. The promoter is only regulated by activation O d. The promoter has multiple operators and multiple enhancers

Q: When researchers analyzed the gad operon they found the following: Expression of the gad operon at…

A: Here we saw that mutant 1 is causing the expression of gad protein even at pH7 which was not…

Q: In the lac operon system, do the inducers act at the transcription or translation level? Explain why…

A: The lactose operon (lac operon) consists of a regulatory gene (i-gene) and three structural genes -…

Q: Figure 16.5 In E. coli, the tip operon is on by default, while the lac operon is off. Why do you…

A: To recap:The operation of the trp and lac operons in E.coli.Introduction:E.coli is a prokaryote with…

Q: The trp repressor binds to the operator region of DNA as a dimer (two repressor protein subunits…

A: The trp operon regulation is a repressible system. When the effector molecule binds to the…

Q: The only way that information is extracted out of DNA is through the transcription of MRNAS that…

A: According to our guideline we can answer only the first question. So upload the second question…

Q: Illustrate simplified region (map) of the lac operon genes. Identify the genes present in this…

A: Lac operon is a control unit that consists of cluster of genes that helps in the metabolism of…

Q: Explain why large amounts of transcript result when a bacterium with a lac Operon is in a medium…

A: Transcription is the process by which RNA is synthesized from DNA. The messenger RNA or mRNA…

Q: Propose a final method of gene regulation of the Up Lateoperon using an updated drawn figure of the…

A: An operon is a part of the DNA where a few genes are clustered together that are transcribed…

Q: IPTG can be used in the laboratory as a synthetic inducer of the lac operon, instead of lactose.…

A: Lactose operon is an example of inducible operon in which the expression of downstream genes will be…

Q: Which of the following statements about the trp operon is false?

A: From above question the statement  If a mutation in region 2 prevents the formation of the 2 + 3…

Q: Consider a mutant of E. coli that has an inactivating mutation in the gene for adenylate cyclase…

A: Inactivating mutations, also known as loss-of-function mutations, cause the gene product to have…

Q: that best fits the phrase below (1-20) Cis-acting B Post-transcriptional modification…

A: Francis Crick proposed central dogma. The RNA is formed from DNA with the help of enzyme RNA…

Q: Considering the fec operon described above, under which conditions would the operon be off? Choose…

A: The protein synthesis process is started from the operon region that is actually worked as a switch.…

Q: The arabinose operon How does the araC protein regulate of the arabinose operon. How does its…

A: How arac protein regulate arabinose operon and how binding differ  If arabinose is absent, the dimer…

Q: Attardi et al (1963) found out that lactose operon mRNA synthesis in E. coli increased with the…

A: Introduction Operon is a genetic regulatory system found in bacteria and their viruses that clusters…

Q: Predict the growth/color pattern you'd expect for constitutive mutants for the lac operon. Table 1:…

A:

Q: The polycistronic Histidine Operon in E. coli encodes all of the proteins necessary for the…

A: An operon is a gene unit of bacterial system whose transcription is positively and negatively…

Q: Describe the main functional differences between the TRP and the Lac operons. Main functional…

A: TRP stands for tryptophan. It is an essential amino acid that must be obtained from the diet and is…

Q: Select the correct statement about the trp operon: high levels of tryptophan promotes high…

A: Introduction :  Lac Operon is a collection of genes with a single promotor that encodes genes for…

Q: Explain how the following conditions will affect the regulation of the operon. Low cAMP levels; E.…

A: Operon is a sequence of DNA that consists of a set of genes which are involved in a particular…

Q: 1) The UR operon which is composed of sequences A, B, C, and D (which may or may not be genes of…

A: An operon may be considered as a group of certain genes which function together so as to regulate…

Q: Which of the following will result in an INCREASE in the levels (# of molecules) of the…

A: Enzyme beta-galactosidase (also known as β- gal) is coded by z  gene of  the lac operon. The…

Q: You are studying the ABCD operon from a newly isolated bacterium. Which of the following would…

A: A Operon consists of segments of DNA , which carry codes for the synthesis of proteins. These genes…

Q: Mutation in the regulatory gene in the lac operon results in the formation of an inactive repressor.…

A: The lac operon is an operon, or group of genes with a single promoter.The genes in the operon encode…

Q: You have created a deletion of the ribosome binding site for the leader sequence of the trp operon.…

A: trp stands for tryptophan operon.In trp operon, there are 5 structural genes viz., trp E, trpD,…

Q: You have created a null mutation of tRNA synthase for trp-tRNA (the synthase enzyme attaches the trp…

A: A null mutation is a mutation that completely removes a gene or part of a gene.In this case, the…

Q: explain this image for the ecoli bacteria mutation trpE

A: Introduction The trp operon is a type of operon system found in E. coli bacteria. The trp operon…

Q: The lactose operon in E. coli comprises two structural genes and three regulatory components.…

A: β-galactosidase activity Permease activity Genotypes Glycerol Lactose Glycerol Lactose A - + -…

Q: Explain how the data shown indicate that two operator sites are necessary for repression of the lac…

A: Introduction: Lac operon exerts positive control on catabolite repression that leads to an increase…

Q: Examine the following theoretical operon (gen) in E. coli which contains structural genes (genC,…

A: Question 1: B. The amino acid is bound to the regulator, causing the regulator to bind to the…

Q: As diagrammed below, some amino acid biosynthetic operons have stem loop structures in the mRNA.…

A: Option A is incorrect as without the formation of heparin the process of translation can not be…

Q: Bacteria with a lac phenotype are unable to use lactose because of some defect in expressing the…

A: Answers - 1) False 2) True 3) True  There are 3 structural genes in lac operon - lac Z, lac Y and…

Q: You mutated the gene encoding the cyclic AMP receptor protein (CRP). In this mutant, the CRP is…

A: Operon is a gene regulatory structure that contains clusters of genes and these clusters of genes…

Q: Refer to figure, which shows the distribution of histone H2A.Z on nucleosomes near a transcription…

A: The DNA is compactly packed in the nucleus because of the presence of the histone proteins which are…

Q: 2. The promoter (P) is the start site of transcription through the binding of RNA polymerase before…

A: The lac operon: The lac operon or the lactose operon is the most widely studied gene with respect to…

Q: Bacterial DNA containing an operon encoding three enzymes is introduced into chromosomal DNA in…

A: Prokaryotes are the organisms that lack the cell nucleus and membrane-bound organelles. They have…

Q: Please study the diagram above on eukaryotic gene expression. In order to provide instructions for…

A: EXPLANATION : Noncoding DNA is also found within most of the eukaryotic genes. Such genes have a…

Q: For the trp operon, determine whether genes will be expressed (on) or silenced (off) given the…

A: Gene is a basic unit of hereditary information or data that fills a fixed position on a chromosome.…

Q: ac operon.  Drag the listed molecules to their corresponding bin. Reset Help   ONPG IPTG X-Gal…

A: ONPG - beta-galactosidase cleaves this molecule to form a visible yellow product. Explaination:  The…

Q: Let’s suppose a DNA mutation changes the consensus sequence at the −35 site in a way that inhibits σ…

A: A sigma factor or specificity factor refers to the protein factor required for the transcription…

Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.