G3A (G at position 3 was changed to A) G9A Deletion of TCA at position 18-20 C22A T31A, A32T double mutant T35G G45C C48A B. What are the promoter sequences of the gene?
The DNA sequence of the promoter region of E. coli xyzA gene is shown below. Transcription start site is the A (in bold) at position 43.
5 10 15 20 25 30 35 40 45 50
GAGCT GTTGA CAATT AATCA TCGAA CTAGT TAACT AGTAC GCAAG TTCAC
Mutations were introduced in the sequence to identify residues important for gene expression. Indicate the effect of the following mutations on xyzA expression (increase, decrease, no effect, cannot be predicted). Provide reasoning for each answer.
A.
- G3A (G at position 3 was changed to A)
- G9A
- Deletion of TCA at position 18-20
- C22A
- T31A, A32T double mutant
- T35G
- G45C
- C48A
B. What are the promoter sequences of the gene?
The Mendelian gene is a basic unit of the heredity and the molecular gene is a sequence of nucleotides present in DNA that is transcribed to produce a functional RNA.
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