The streptolysin S toxin made by S. pyogenes is encoded by a 9-geneoperon, sagABCDEFGHI. Thinking about what a 3-line diagram would look like for thisoperon, answer the following questions. Write numeric answers only. For example, if youranswer is 6 promoters, write only 6.1) How many promoters control the expression of these genes?2) How many locations does RNA Polymerase bind to get full expression of these genes?3) How many ribosome binding sites are needed for full protein expression?4) How many start codons will be needed for full protein expression?5) How many mRNA strands will be produced with full operon expression?6) How many proteins will be produced with full protein expression?1

Name: The streptolysin S toxin made by S. pyogenes is encoded by a 9-geneop
Uploaded: 2024-03-20T06:42:41.000Z
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Description: The streptolysin S toxin made by S. pyogenes is encoded by a 9-geneoperon, sagABCDEFGHI. Thinking about what a 3-line diagram would look like for thisoperon, answer the following questions. Write numeric answers only. For example, if youranswer is 6

An operon is a unit of genetic regulation found in prokaryotic organisms like bacteria and archaea. It consists of a cluster of genes that are transcribed together into a single mRNA molecule. This mRNA carries the information to produce multiple proteins involved in related functions.The operon typically includes the following components:Promoter: A region of DNA where RNA polymerase binds to initiate transcription of the genes within the operon.Operator: A regulatory DNA sequence located near the promoter. It serves as a binding site for a repressor protein, which can inhibit or regulate the transcription of the genes in the operon by blocking RNA polymerase from binding to the promoter.Structural Genes: These are the genes that encode proteins with related functions. They are transcribed together into a single mRNA molecule, allowing coordinated expression of the proteins they encode.Regulatory Genes (optional): Some operons contain an additional gene that codes for a regulatory protein, such as a repressor or an activator. This protein can bind to the operator sequence and control the expression of the structural genes by either facilitating or blocking transcription.Since all 9 genes are part of a single operon, number of promoters is 1. RNA polymerase binds to the promoter sequence to initialize the transcription process. As there is only one promoter, RNA polymerase will bind to 1 location for full expression of these genes. Within an operon, each gene typically has its own RBS sequence, located in the mRNA immediately upstream of the start codon (usually AUG) of the respective gene. So, 9 ribosome binding sites will be present for 9 genes in this case. All the genes should have their own start codon. So there will be 9 start codons for full protein expression.For full protein expression, a single mRNA strand will be formed as all the genes are under the same transcription machinery. 9 proteins will be produced from full operon expression. Number of promoter -1. RNA polymerase binding location- 1 Ribosome binding sites -9. Start codon- 9mRNA strand- 1. Number of protein- 9.