Chromatin Immunoprecipitation (ChIP) experiments enable researchers to measure the levels of transcription factors, coactivators/corepressors and chromatin remodeling complexes on a specific gene in cells. In the ChIP experiment below, the recruitment of the transcription factor NfkB, Histone Deacetylase Complex (HDAC3) and p300/CBP complex to the Interleukin 12 (IL12) gene at various times (0, 30, 60 and 120 min) after treating cells with LPS are measured. Does LPS stimulate or inhibit transcription of the IL12 gene based on the recruitment of factors to the IL12 gene.
Chromatin Immunoprecipitation (ChIP) experiments enable researchers to measure the levels of transcription factors, coactivators/corepressors and chromatin remodeling complexes on a specific gene in cells. In the ChIP experiment below, the recruitment of the transcription factor NfkB, Histone Deacetylase Complex (HDAC3) and p300/CBP complex to the Interleukin 12 (IL12) gene at various times (0, 30, 60 and 120 min) after treating cells with LPS are measured. Does LPS stimulate or inhibit transcription of the IL12 gene based on the recruitment of factors to the IL12 gene.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Chromatin Immunoprecipitation (ChIP) experiments enable researchers to measure the levels of transcription factors, coactivators/corepressors and chromatin remodeling complexes on a specific gene in cells. In the ChIP experiment below, the recruitment of the transcription factor NfkB, Histone Deacetylase Complex (HDAC3) and p300/CBP complex to the Interleukin 12 (IL12) gene at various times (0, 30, 60 and 120 min) after treating cells with LPS are measured. Does LPS stimulate or inhibit transcription of the IL12 gene based on the recruitment of factors to the IL12 gene. Justify your answer by explaining the state of the gene before LPS and after LPS treatment (limit 5-6 sentences).
Transcribed Image Text:IP
NFkB
HDAC3
p300
IgG
Input
WT
0 30 60 120
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