Plasmid +1 -789 Luc -419 Luc -309 = HLuc -239 Luc --199 Luc --149 Luc -123 Luc -59 = Luc -15%D Luc Luc Luc + + + 2 4 6. 10 34 Relative Luciferase Activity PCD3Y-789. PCD3Y-419, PCD3Y-309. PCD3Y-239. PCD3Y-199, PCD3Y-149, PCD3Y-123, PCD3Y-59. PCD3Y-15. Parent Plasmid, Parent Plasmid. with non-CD3Y Active Promoter +1 Transcribed Region Upstream Regulatory Sequences CD3Y Gene Plasmid Introduce Plasmids into T Lymphocytes Location in Which CD3Y Sequences Are Inserted Luciferase Gene Luciferase Production Poly (A) Signal T Lymphocytes with Plasmids Plasmid
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
CD3 is a signaling protein that is typically found only in the plasma membrane of immune system T lymphocytes. CD3 is composed of several different polypeptides, including a gamma chain, CD3γ. Scientists analyzed the promoter of the CD3γ chain gene for regulatory sequences that might have positive or negative effects on expression of the gene.
The scientists cloned fragments of the CD3γ gene that included the first transcribed
(a) Identify both the cellular component and the location of the component that is responsible for producing the luciferase protein from mRNAs transcribed in the plasmid-containing T lymphocytes. Explain what dictates to the lymphocytes the correct order in which amino acids should be linked to form the luciferase protein.
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