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c) You use chromatin immunoprecipitation to measure the location and amount of transcription factors binding around the Lrg1 gene in the four treatments. You use your data to generate the following figure. Orient yourself to the figure by finding the transcription start site on the x-axis. The size of the peak indicates the frequency of the transcription factor at that position. Lrg1 Lrg1 transcription Control . = 12 2. 3 Sucrose = 77 Sucrose + = 180 Hormone 4 Starvation = 2 -2,000 bp -1,000 bp +1 bp +1,000 bp What type of transcription factor might be present at position 2? What evidence i) from the figure supports your claim? (two sentences max) ii) What type of transcription factor might be present at position 3? What evidence from the figure supports your claim? (two sentences max) ii) Look at the transcription factors at position 4. Use evidence from the figure to explain the effect they are having on transcription of Lrg1. (two sentences max)

c) You use chromatin immunoprecipitation to measure the location and amount of transcription factors binding around the Lrg1 gene in the four treatments. You use your data to generate the following figure. Orient yourself to the figure by finding the transcription start site on the x-axis. The size of the peak indicates the frequency of the transcription factor at that position. Lrg1 Lrg1 transcription Control . = 12 2. 3 Sucrose = 77 Sucrose + = 180 Hormone 4 Starvation = 2 -2,000 bp -1,000 bp +1 bp +1,000 bp What type of transcription factor might be present at position 2? What evidence i) from the figure supports your claim? (two sentences max) ii) What type of transcription factor might be present at position 3? What evidence from the figure supports your claim? (two sentences max) ii) Look at the transcription factors at position 4. Use evidence from the figure to explain the effect they are having on transcription of Lrg1. (two sentences max)

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Transcription: iv) What type of transcription factors might be present at position 6? What evidence from the figure supports your claim? (two sentences max) v) Based on the figure, propose a hypothesis for how addition of hormone affects transcription of Lrg1, including the specific type of transcription factors triggered by the hormone and what they do. (two sentences max) vi) Explain why a transcription factor binds to some DNA sequences but not others. (two sentences max)
Transcribed Image Text:Transcription: iv) What type of transcription factors might be present at position 6? What evidence from the figure supports your claim? (two sentences max) v) Based on the figure, propose a hypothesis for how addition of hormone affects transcription of Lrg1, including the specific type of transcription factors triggered by the hormone and what they do. (two sentences max) vi) Explain why a transcription factor binds to some DNA sequences but not others. (two sentences max)
The image shows results from a chromatin immunoprecipitation experiment measuring transcription factor binding around the Lrg1 gene under four different treatments: Control, Sucrose, Sucrose + Hormone, and Starvation. The x-axis represents the distance in base pairs (bp) from the transcription start site of the Lrg1 gene, with the scale ranging from -2000 bp to +1000 bp. Peaks in the graphs represent transcription factor binding frequency at specific genomic locations. **Graphs:** 1. **Control:** - Peaks are observed notably at position 1. - Lrg1 transcription level is given as 12. 2. **Sucrose:** - Peaks observed at positions 2 and 3. - Lrg1 transcription level is 77. 3. **Sucrose + Hormone:** - Prominent peaks at positions 2 and 3, larger than in sucrose-only treatment. - Lrg1 transcription level is 180. 4. **Starvation:** - Peaks are noted at positions 4, 5, and a less intense one at 6. - Lrg1 transcription level is 2. **Questions:** i) At position 2, the transcription factor might function as an enhancer. Evidence for this is the elevated peaks and increased Lrg1 transcription level under the Sucrose and Sucrose + Hormone conditions, compared to the Control. ii) Position 3 likely involves an upstream activator transcription factor as there is a strong peak in the Sucrose and Sucrose + Hormone conditions. This coincides with a higher transcription level of Lrg1 compared to the Control condition. iii) Transcription factors at position 4 in the Starvation condition seem to act as repressors, given the decreased transcription of Lrg1 (2) compared to other conditions. This is supported by the presence of peaks without an increase in Lrg1 transcription.
Transcribed Image Text:The image shows results from a chromatin immunoprecipitation experiment measuring transcription factor binding around the Lrg1 gene under four different treatments: Control, Sucrose, Sucrose + Hormone, and Starvation. The x-axis represents the distance in base pairs (bp) from the transcription start site of the Lrg1 gene, with the scale ranging from -2000 bp to +1000 bp. Peaks in the graphs represent transcription factor binding frequency at specific genomic locations. **Graphs:** 1. **Control:** - Peaks are observed notably at position 1. - Lrg1 transcription level is given as 12. 2. **Sucrose:** - Peaks observed at positions 2 and 3. - Lrg1 transcription level is 77. 3. **Sucrose + Hormone:** - Prominent peaks at positions 2 and 3, larger than in sucrose-only treatment. - Lrg1 transcription level is 180. 4. **Starvation:** - Peaks are noted at positions 4, 5, and a less intense one at 6. - Lrg1 transcription level is 2. **Questions:** i) At position 2, the transcription factor might function as an enhancer. Evidence for this is the elevated peaks and increased Lrg1 transcription level under the Sucrose and Sucrose + Hormone conditions, compared to the Control. ii) Position 3 likely involves an upstream activator transcription factor as there is a strong peak in the Sucrose and Sucrose + Hormone conditions. This coincides with a higher transcription level of Lrg1 compared to the Control condition. iii) Transcription factors at position 4 in the Starvation condition seem to act as repressors, given the decreased transcription of Lrg1 (2) compared to other conditions. This is supported by the presence of peaks without an increase in Lrg1 transcription.
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