DMC1 SPS1 DIT1 SPS100 fold repressed >20 1:1 fold induced x10x >20

Human Anatomy & Physiology (11th Edition)
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ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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a)
hours: 0 2 567
DMC1
SPS1
hours: 0 2 5 6 7 9 11
DIT1
SPS100
Oxygen level (% normal)
b)
120
100
FIGURE 4.6. Comparison of Northern blots with DNA microarray
data. a) Results from four individual Northern blots examining four different
genes and measuring mRNA production over time, as indicated. b) Results
from a series of microarrays for the same four genes of interest. Note the color
scale on the bottom of b), where bright green indicates a 20-fold repression
and bright red indicates a 20-fold induction. Black indicates no change in
transcription (i.e., the merged microarray spot would have appeared yellow).
80
60
40
20
0 1 2
gene X
gene Y
gene Z
3
1
hour
1.0
1.0
b)
1.0
hours: 0.5 2 5 7 9 11
DMC1
SPS1
DIT1
SPS100
fold repressed
>20 10x 3x3x10x >20
1:1
fold induced
4 5 6 7 8 9 10 11
Time (hours)
3
hour
2.2
4.5
1.5
5
9
hour
hour
1.0
0.15
0.95 0.05
2.0
2.0
FIGURE 4.7 Transcriptional response of three genes
to the gradual loss of oxygen. a) Graph of oxygen con-
sumption over time by yeast growing in a closed container.
b) DNA microarray data given in the form of ratios. To cal
culate a ratio, one gene's activity in cells gradually consum-
ing all the available oxygen was divided by its activity in
control cells with unlimited oxygen.
Transcribed Image Text:a) hours: 0 2 567 DMC1 SPS1 hours: 0 2 5 6 7 9 11 DIT1 SPS100 Oxygen level (% normal) b) 120 100 FIGURE 4.6. Comparison of Northern blots with DNA microarray data. a) Results from four individual Northern blots examining four different genes and measuring mRNA production over time, as indicated. b) Results from a series of microarrays for the same four genes of interest. Note the color scale on the bottom of b), where bright green indicates a 20-fold repression and bright red indicates a 20-fold induction. Black indicates no change in transcription (i.e., the merged microarray spot would have appeared yellow). 80 60 40 20 0 1 2 gene X gene Y gene Z 3 1 hour 1.0 1.0 b) 1.0 hours: 0.5 2 5 7 9 11 DMC1 SPS1 DIT1 SPS100 fold repressed >20 10x 3x3x10x >20 1:1 fold induced 4 5 6 7 8 9 10 11 Time (hours) 3 hour 2.2 4.5 1.5 5 9 hour hour 1.0 0.15 0.95 0.05 2.0 2.0 FIGURE 4.7 Transcriptional response of three genes to the gradual loss of oxygen. a) Graph of oxygen con- sumption over time by yeast growing in a closed container. b) DNA microarray data given in the form of ratios. To cal culate a ratio, one gene's activity in cells gradually consum- ing all the available oxygen was divided by its activity in control cells with unlimited oxygen.
Look at Figure 4.7, which depicts the loss of oxygen over time and the transcriptional
response of three genes. These data are the ratios of transcription for genes X, Y, and Z
during the depletion of oxygen.
a. What is the fold change for each gene at each time point?
b. Using the color scale from Figure 4.6, determine the color for each ratio in Figure
4.7b.
c. Were any of the genes in Figure 4.7b transcribed similarly?
Transcribed Image Text:Look at Figure 4.7, which depicts the loss of oxygen over time and the transcriptional response of three genes. These data are the ratios of transcription for genes X, Y, and Z during the depletion of oxygen. a. What is the fold change for each gene at each time point? b. Using the color scale from Figure 4.6, determine the color for each ratio in Figure 4.7b. c. Were any of the genes in Figure 4.7b transcribed similarly?
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