Gene Actin (housekeeping gene) Annexin I c. You hybridize the cDNA to your microarrays, scan your microarrays and put the data into a graphical representation (heat map). Fill out the following table based on the expression ratios given. CD81 antigen Rho GDI Maspin P Expression Ratio 1.0 11.7 0.35 0.2 23.0 Induced or repressed? Color of spot on microarray Color in graph

Q: Describe the banding pattern seen in the gel. What does that tell you about the role of annealing…

A: Gel electrophoresis is a laboratory technique for separating charged molecules such as DNA, RNA, and…

Q: Why isn’t cDNA synthetic

A: INTRODUCTION Deoxyribonucleic Acid, or DNA, is a molecule that holds the instructions that an…

Q: For each of the following items, describe what it is explain the necessity for each in cloning…

A: Genetic engineering is the application of technology in the manipulation of genetic information.…

Q: Please select appropriate word in each bracket CRISPR-Cas9 is a system for directly changing the…

A: Genetic material is nothing but the sequence of nucleic acids which is called as DNA. It contains…

Q: Why are restriction endonucleases considered a bacteria’s “innate immune system”? Why is…

A: Restriction endonucleases, also known as restriction enzymes, are enzymes that are found in bacteria…

Q: 5' 3' ORF1 ORF2 ORF3 ORF4 ORF5 1. Above are pictured 2 operons, one that includes ORF1 and OFR2 and…

A: The production of RNA from DNA is known as transcription that occurs within the cytoplasm of…

Q: During conjugation,. HFR; F+ function as DNA donors and, function as DNA recipients. F+; F- F-; F+…

A: Conjugation is a type of horizontal gene transfer seen in prokaryotes.

Q: If a point substitution mutation changes the sequence 5'ATGAAA3' to 5'ACGAAA3' in the MIDDLE of the…

A: Point mutation is a genetic mutation in which a single nucleotide base is altered, inserted or…

Q: "The design of trans-Tango is modular, so it is not limited to tracing experiments. Since the…

A: It is certainly possible to further advance the trans-Tango system to label third-order neurons,…

Q: How long is the polypeptide produced from the following mRNA transcript?…

A: We can find this answer using a codon chart- 5-UCA UGC UUG GAC UCA AGU CUA CGU GAA U-3' As each mRNA…

Q: You ligate a CDNA fragment with Bgl Il and Xho I sticky ends into the Bgl Il and Xho l sites of the…

A: Given that  *Ligated a CDNA fragment with Bgl II and Xho I into Bgl II and Xho I sites  respectively…

Q: Given the Following DNA template, TAC   CGC   TCC   GCC  GTC   GAC   AAT   ACC   ACT, write out the…

A: The DNA template is used for the synthesis of mRNA molecules. mRNA molecule is used by the…

Q: For tailed PCR primers the "tails" can have sequence that is NOT complementary to the target…

A: PCR - (polymerase chain reaction) is used to amplify the DNA sequence through repetitive cycles. It…

Q: Why and how do the polymerase go backward from state 2 (post-translocation state) to state…

A: During replication and transcription, the polymerase enzyme moves along the DNA template to…

Q: Based on the following wild type DNA sequence, indicate if each of the mutations should be…

A: A mutation is a change in a DNA sequence. Mutations can result from DNA copying mistakes made during…

Q: The 1000 aa protein (Protein X) you are studying has several putative caspase cleavage sites. You…

A: Western blotting is a molecular biology technique that is used to detect a specific protein from a…

Q: Transposons in bacterial genomes can carry genes,including those conferring ________ resistance

A: The transposable elements are known as stretches of DNA molecules that can jump from one place to…

Q: Discuss the use of reverse transcriptase in theconstruction of a cDNA library

A: The collection of cloned cDNA fragments constitute a cDNA library. It contains only DNA that codes…

Q: RNA polymerases

A: RNA polymerase:It is an important enzyme involved in transcription of DNA to RNA, gene expression.It…

Q: Refer to the lac operon image above to see the available choices. Which lettered item represents the…

A: A set of genes present in E.coli. that regulates the uptake of lactose from the surrounding…

Q: The presence of a DNA template (e.g. a product from PCR), general transcription factors, and RNA…

A: To answer this question, let us first address the main difference between genomic DNA and the PCR…

Q: b. Which one of the following a cell mutants will be able to switch at least once? [Select]

A: Alpha (α) thalassemia is a genetic problem and is brought about by cancellations or transformations…

Q: would the J3480 Code and 59 Modifer Code go on seperate lines under the HCPCS Level II codes column?

A: HCPCS Code: J3480 - Injection, Marcaine, 30 mlModifier: 59 - Distinct procedural service (to…

Q: 汽 (0 hope this picture, https://www.phys.kSu.edu/gene/photos/sd.html. 2 will help you imagine the…

A: Isolation is a microbiological technique in which individual bacterial colonies are separated from a…

Q: The two strands of DNA are held together by. bonds, but can be denatured by and The nucleotides are…

A: Note - Since you have asked multiple questions, we will solve the first question for you. If you…

Q: Can you explain what is nucleotide selectivity in polymerase

A: One of the structural elements, or building blocks, of DNA and RNA is the nucleotide. A nucleotide…

Q: What is lac operon system?

A: Lac operon is one of the method by which a prokaryotic cell control gene expression.  The lac operon…

Q: What is Taq polymerase and why is it important?

A: DNA Polymerase is a very important enzyme in the process of DNA Replication. It is a process in…

Q: 5' ATGATCTCGTAA 3' 3' TACTAGAGCATT 5'

A: DNA, or deoxyribonucleic acid, is a hereditary molecule that encodes the blueprint for the growth,…