Please select appropriate word in each bracket CRISPR-Cas9 is a system for directly changing the sequence of [ Select ] ["DNA", "RNA"] and is derived from bacterial systems designed to block infection by [ Select ] ["bacteriophage", "HIV"] . The original system contained two RNA molecules, crRNA and tracrRNA, that formed a complex that recruited Cas9 protein. Dr. Doudna and her colleague Dr. Emmanuelle Charpentier found they could make a single guide RNA (sgRNA) that had an [ Select ] ["intramolecular interaction (H-bonding)", "RNAse activity"] that could bind and activate Cas9 to form a break at the sequence targeted by the sgRNA. While the CRISPR-Cas9 system can direct scission of the target, any change in sequence is achieved by [ Select ] ["the cells endogenous repair system", "providing cells with DNA recombination proteins"] . Therefore, generating small deletions or insertions is technically [ Select ] ["more difficult", "more feasible"] than precise replacement of a specific sequence.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Please select appropriate word in each bracket
CRISPR-Cas9 is a system for directly changing the sequence of [ Select ] ["DNA", "RNA"] and is derived from bacterial systems designed to block infection by [ Select ] ["bacteriophage", "HIV"] . The original system contained two RNA molecules, crRNA and tracrRNA, that formed a complex that recruited Cas9 protein. Dr. Doudna and her colleague Dr. Emmanuelle Charpentier found they could make a single guide RNA (sgRNA) that had an [ Select ] ["intramolecular interaction (H-bonding)", "RNAse activity"] that could bind and activate Cas9 to form a break at the sequence targeted by the sgRNA. While the CRISPR-Cas9 system can direct scission of the target, any change in sequence is achieved by [ Select ] ["the cells endogenous repair system", "providing cells with DNA recombination proteins"] . Therefore, generating small deletions or insertions is technically [ Select ] ["more difficult", "more feasible"] than precise replacement of a specific sequence.
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