QUESTION 8 Why did you bother to identify the introns? O So that I could include them in the sequence to understand intron function. O So that I could exclude them from the sequence because prokaryotes don't have spliceosomal machinery. O So that I could see how introns affect protein folding.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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I only need help with question #8.

Use question #7 as context for question #8.

QUESTION 7
Great! Now you have the gene sequence. Now you would like to clone it into an expression vector to grow up in a bacterial system. Because you're going to use bacteria to generate protein from a
eukaryote, the mammoth, you need to get rid of introns from your sequence. How do you do that?
O Bioinformatically, I look for splice-site sequences and branch-point adenines and predict intron-exon boundaries
O l use a comparative genomic approach and use sequence homology with the genome of a closely related species
O l use a comparative genomic approach and use sequence homology with the genome of a distantly related species
O Both A and B
O Both B and C
QUESTION 8
Why did you bother to identify the introns?
O So that I could include them in the sequence to understand intron function.
O So that I could exclude them from the sequence because prokaryotes don't have spliceosomal machinery.
O So that I could see how introns affect protein folding.
Transcribed Image Text:QUESTION 7 Great! Now you have the gene sequence. Now you would like to clone it into an expression vector to grow up in a bacterial system. Because you're going to use bacteria to generate protein from a eukaryote, the mammoth, you need to get rid of introns from your sequence. How do you do that? O Bioinformatically, I look for splice-site sequences and branch-point adenines and predict intron-exon boundaries O l use a comparative genomic approach and use sequence homology with the genome of a closely related species O l use a comparative genomic approach and use sequence homology with the genome of a distantly related species O Both A and B O Both B and C QUESTION 8 Why did you bother to identify the introns? O So that I could include them in the sequence to understand intron function. O So that I could exclude them from the sequence because prokaryotes don't have spliceosomal machinery. O So that I could see how introns affect protein folding.
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