"The design of trans-Tango is modular, so it is not limited to tracing experiments. Since the readout of trans-Tango is transcription of a reporter gene, the system can be readily configured for functional experiments in which the activity of second-order neurons is altered (e.g., by incorporating optogenetic proteins as the reporter) or monitored (e.g., by incorporating calcium sensors as the reporter)." If there's NO genetic restriction (that means you can put as many trans- genetic elements in the target fly), is it possible to further advance the trans-Tango system, making the system capable of labeling "the third-order" neurons (means the neurons that connect with the second- order neurons; aka down-stream of the second-order neurons)? What additional genetic elements are required? Please give your best shot to answer these questions.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Plz asap with conclusion
"The design of trans-Tango is modular, so it
is not limited to tracing experiments. Since
the readout of trans-Tango is transcription
of a reporter gene, the system can be
readily configured for functional
experiments in which the activity of
second-order neurons is altered (e.g., by
incorporating optogenetic proteins as the
reporter) or monitored (e.g., by
incorporating calcium sensors as the
reporter)." If there's NO genetic restriction
(that means you can put as many trans-
genetic elements in the target fly), is it
possible to further advance the trans-Tango
system, making the system capable of
labeling "the third-order" neurons (means
the neurons that connect with the second-
order neurons; aka down-stream of the
second-order neurons)? What additional
genetic elements are required? Please give
your best shot to answer these questions.
Transcribed Image Text:"The design of trans-Tango is modular, so it is not limited to tracing experiments. Since the readout of trans-Tango is transcription of a reporter gene, the system can be readily configured for functional experiments in which the activity of second-order neurons is altered (e.g., by incorporating optogenetic proteins as the reporter) or monitored (e.g., by incorporating calcium sensors as the reporter)." If there's NO genetic restriction (that means you can put as many trans- genetic elements in the target fly), is it possible to further advance the trans-Tango system, making the system capable of labeling "the third-order" neurons (means the neurons that connect with the second- order neurons; aka down-stream of the second-order neurons)? What additional genetic elements are required? Please give your best shot to answer these questions.
UAS-HGCG:hICAM1NRXN1
10XUAS intron syn21 hGCG
trans-Tango
flexible linker
with myc-tag
hICAM1
Syb Promoter DSCP hGCGR TEVES OF
Elav Promoter Arrestin2 TEV Protease SV40 polyA
dNeurexint
hsp70 poly
p10 poly
Transcribed Image Text:UAS-HGCG:hICAM1NRXN1 10XUAS intron syn21 hGCG trans-Tango flexible linker with myc-tag hICAM1 Syb Promoter DSCP hGCGR TEVES OF Elav Promoter Arrestin2 TEV Protease SV40 polyA dNeurexint hsp70 poly p10 poly
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