7. Flow of carbon atoms. What is the fate of the radioac- tive label when each of the following compounds is added to a cell extract containing the enzymes and cofac- tors of the glycolytic pathway, the citric acid cycle, and the pyruvate dehydrogenase complex? (The 14C label is printed in red.) (a) (c) H₂C H3C O C. O COO™ COO (b) (d) H3C H₂C O C. O C. (e) Glucose 6-phosphate labeled at C-1. COO™ S-COA
7. Flow of carbon atoms. What is the fate of the radioac- tive label when each of the following compounds is added to a cell extract containing the enzymes and cofac- tors of the glycolytic pathway, the citric acid cycle, and the pyruvate dehydrogenase complex? (The 14C label is printed in red.) (a) (c) H₂C H3C O C. O COO™ COO (b) (d) H3C H₂C O C. O C. (e) Glucose 6-phosphate labeled at C-1. COO™ S-COA
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
Transcribed Image Text:7. Flow of carbon atoms. What is the fate of the radioac-
tive label when each of the following compounds is
added to a cell extract containing the enzymes and cofac-
tors of the glycolytic pathway, the citric acid cycle, and
the pyruvate dehydrogenase complex? (The ¹4C label is
printed in red.)
(a)
(c)
H3C
H3C
O
C.
O
C.
COO
COO-
(b)
(d)
H3C
H₂C
O
C.
O
C.
(e) Glucose 6-phosphate labeled at C-1.
COO™
S-COA
Expert Solution
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Six-carbon glucose enters the glycolytic pathway where it is converted into two molecules of 3 carbon pyruvate in a series of enzymatic reactions. Pyruvate is then converted to acetyl CoA by pyruvate dehydrogenase complex. Acetyl CoA enters the TCA cycle by reacting with oxaloacetate.
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In follow up would it be possible to do part D and E in the question as well?
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