You ligate a CDNA fragment with Bgl Il and Xho I sticky ends into the Bgl Il and Xho l sites of the multi-cloning site within a vector (vector plus insert= 9 kbp). The vector has a resistance gene for kanamycin. Following the ligation, you transform the vector + insert (aka plasmid DNA) into chemically competent bacteria and spread the transformed bacteria on LB agar plates with antibiotic. The plates are incubated overnight at 37°C. If you spread the transformed bacteria on LB agar plates with ampicillin, what would you expect to see on the plate following a 24h incubation? Select one or more: V a. no bacteria/no growth O b. bacteria with vector + insert O c. insert alone O d. bacteria with vector alone O e. bacteria with no vector

Q: O 100% O 75% O 50% O 0%

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