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You ligate a CDNA fragment with Bgl Il and Xho I sticky ends into the Bgl Il and Xho l sites of the multi-cloning site within a vector (vector plus insert= 9 kbp). The vector has a resistance gene for kanamycin. Following the ligation, you transform the vector + insert (aka plasmid DNA) into chemically competent bacteria and spread the transformed bacteria on LB agar plates with antibiotic. The plates are incubated overnight at 37°C. If you spread the transformed bacteria on LB agar plates with ampicillin, what would you expect to see on the plate following a 24h incubation? Select one or more: V a. no bacteria/no growth O b. bacteria with vector + insert O c. insert alone O d. bacteria with vector alone O e. bacteria with no vector

You ligate a CDNA fragment with Bgl Il and Xho I sticky ends into the Bgl Il and Xho l sites of the multi-cloning site within a vector (vector plus insert= 9 kbp). The vector has a resistance gene for kanamycin. Following the ligation, you transform the vector + insert (aka plasmid DNA) into chemically competent bacteria and spread the transformed bacteria on LB agar plates with antibiotic. The plates are incubated overnight at 37°C. If you spread the transformed bacteria on LB agar plates with ampicillin, what would you expect to see on the plate following a 24h incubation? Select one or more: V a. no bacteria/no growth O b. bacteria with vector + insert O c. insert alone O d. bacteria with vector alone O e. bacteria with no vector

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You ligate a CDNA fragment with Bgl Il and Xho I sticky ends into the Bgl Il and Xho I sites of the multi-cloning site within a vector (vector plus insert= 9 kbp). The vector has a resistance gene for kanamycin. Following the ligation, you transform the vector + insert (aka plasmid DNA) into chemically competent bacteria and spread the transformed bacteria on LB agar plates with antibiotic. The plates are incubated overnight at 37°C. If you spread the transformed bacteria on LB agar plates with ampicillin, what would you expect to see on the plate following a 24h incubation? Select one or more: V a. no bacteria/no growth b. bacteria with vector + insert O C. insert alone O d. bacteria with vector alone е. bacteria with no vector
Transcribed Image Text:You ligate a CDNA fragment with Bgl Il and Xho I sticky ends into the Bgl Il and Xho I sites of the multi-cloning site within a vector (vector plus insert= 9 kbp). The vector has a resistance gene for kanamycin. Following the ligation, you transform the vector + insert (aka plasmid DNA) into chemically competent bacteria and spread the transformed bacteria on LB agar plates with antibiotic. The plates are incubated overnight at 37°C. If you spread the transformed bacteria on LB agar plates with ampicillin, what would you expect to see on the plate following a 24h incubation? Select one or more: V a. no bacteria/no growth b. bacteria with vector + insert O C. insert alone O d. bacteria with vector alone е. bacteria with no vector
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