Refer to the lac operon image above to see the available choices. Which lettered item represents the DNA sequence where repressor binds? O E

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### lac Operon Induction Mechanism

The diagram explains the induction mechanism of the lac operon in prokaryotic cells. The lac operon is a cluster of genes responsible for the transport and metabolism of lactose in Escherichia coli (E. coli) and other bacteria. Below are the detailed components of the diagram:

#### Components:

**A: Structural Genes (lacZ, lacY, lacA)**
- **lacZ**: Encodes β-galactosidase, an enzyme that breaks down lactose into glucose and galactose.
- **lacY**: Encodes lactose permease, a protein involved in the transport of lactose into the cell.
- **lacA**: Encodes thiogalactoside transacetylase, an enzyme with a role in detoxification (exact function in lactose metabolism is less clear).

**B: Allolactose**
- A metabolite of lactose that acts as an inducer of the lac operon.
- Binds to the lacI repressor and inactivates it, preventing the repressor from binding to the operator.

**C: lacI**
- Represents the repressor protein encoded by the lacI gene.
- In the absence of allolactose, the repressor binds to the operator (D), inhibiting transcription of the structural genes.

**D: Operator**
- A DNA sequence to which the lacI repressor binds.
- When the repressor is bound, it prevents RNA polymerase from transcribing the structural genes.

**E: CAP (Catabolite Activator Protein)**
- Activates transcription in the presence of cAMP.
- Binds to the CAP site near the promoter and enhances the binding of RNA polymerase to the promoter, increasing transcription of the lac operon.

#### Pathway of Induction:
1. **Lactose Entry and Metabolism**: Lactose enters the cell and is converted into allolactose.
2. **Allolactose Binding**: Allolactose binds to the lacI repressor, causing a conformational change that releases the repressor from the operator region (D).
3. **Transcription Activation**: Without the repressor binding the operator, RNA polymerase can transcribe the structural genes (A), leading to the production of β-galactosidase, lactose permease, and thiogalactoside transacetylase.
4. **CAP Function**
Transcribed Image Text:### lac Operon Induction Mechanism The diagram explains the induction mechanism of the lac operon in prokaryotic cells. The lac operon is a cluster of genes responsible for the transport and metabolism of lactose in Escherichia coli (E. coli) and other bacteria. Below are the detailed components of the diagram: #### Components: **A: Structural Genes (lacZ, lacY, lacA)** - **lacZ**: Encodes β-galactosidase, an enzyme that breaks down lactose into glucose and galactose. - **lacY**: Encodes lactose permease, a protein involved in the transport of lactose into the cell. - **lacA**: Encodes thiogalactoside transacetylase, an enzyme with a role in detoxification (exact function in lactose metabolism is less clear). **B: Allolactose** - A metabolite of lactose that acts as an inducer of the lac operon. - Binds to the lacI repressor and inactivates it, preventing the repressor from binding to the operator. **C: lacI** - Represents the repressor protein encoded by the lacI gene. - In the absence of allolactose, the repressor binds to the operator (D), inhibiting transcription of the structural genes. **D: Operator** - A DNA sequence to which the lacI repressor binds. - When the repressor is bound, it prevents RNA polymerase from transcribing the structural genes. **E: CAP (Catabolite Activator Protein)** - Activates transcription in the presence of cAMP. - Binds to the CAP site near the promoter and enhances the binding of RNA polymerase to the promoter, increasing transcription of the lac operon. #### Pathway of Induction: 1. **Lactose Entry and Metabolism**: Lactose enters the cell and is converted into allolactose. 2. **Allolactose Binding**: Allolactose binds to the lacI repressor, causing a conformational change that releases the repressor from the operator region (D). 3. **Transcription Activation**: Without the repressor binding the operator, RNA polymerase can transcribe the structural genes (A), leading to the production of β-galactosidase, lactose permease, and thiogalactoside transacetylase. 4. **CAP Function**
### Question: Lac Operon DNA Sequence Binding Site

#### Refer to the *lac* operon image above to see the available choices. Which lettered item represents the DNA sequence where the repressor binds?

**Options:**
- O E
- O A
- O B
- O C
- O D

*Note: The referenced lac operon image should depict various components labeled with different letters, indicating specific sequences or regions within the operon.*
Transcribed Image Text:### Question: Lac Operon DNA Sequence Binding Site #### Refer to the *lac* operon image above to see the available choices. Which lettered item represents the DNA sequence where the repressor binds? **Options:** - O E - O A - O B - O C - O D *Note: The referenced lac operon image should depict various components labeled with different letters, indicating specific sequences or regions within the operon.*
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