Q: a. What are the purposes of sequence alignment?
A: Sequence alignment is a method by which one can arrange sequences of DNA, RNA and protein so as to…
Q: What is the general effect of methylation of a genomics region on transcription?
A: DNA methylation is a biochemical process involving the addition of methyl groups to the Dna strand.…
Q: Define origin-recognition complex (ORC)
A: The field of biology that studies the composition, and structure of the molecules and also the…
Q: What is the ENCODE project?
A: ENCODE is Encyclopedia of DNA Elements. It is a project funded by National Human Genome Research…
Q: What are the requirements for an expression vector?
A: The expression vector helps to yield the product of the cloned gene.
Q: How big (in base pairs) is (are) the fragments generated by digesting the vector with EcoRI
A: Base pairs - there are four bases or nucleotides in DNA: ADENINE, CYTOSINE, GUANINE, and thymine.
Q: What would be the modifier to add to this HCPCS code?
A: HCPCS Level II codes are a combination of alphabets and numerals assigned to medical services,…
Q: What is meant by Genetic Information NondiscriminationAct (GINA) ?
A: Genetics can be defined as the branch of biology which is concerned with the study of genes, genetic…
Q: What separates the base pair at the start of DNAreplication?
A: DNA replication is the process in which DNA makes a copy of itself by copying a double-stranded DNA…
Q: In transcription, what is/are the importance of the series of nucleotide bases before the coding…
A: The coding region before transcription flanked by promoter sequence on 5' end on the template strand…
Q: What is expressed sequence tag (EST)?
A: The sequencing of the genome from different organisms has been performed for many years due to many…
Q: Give the sequence on the opposite strand for ACGTAT, AGATCT, and ATGGTA (all read 5' to 3'). Are the…
A: Deoxyribonucleic acid (DNA) and Ribonucleic acid (RNA) are the two types of nucleic acids forming…
Q: What is a telomere problem and how is telomerase useful in this context?
A: Telomere is the sequence of repetitive nucleotides present at the ends of the chromosomes. Telomeres…
Q: For insertion elements and simple transposons, what is the functionof the inverted repeat sequences…
A: The sequence of DNA (deoxyribonucleic acid) that changes the position in the genome, and at times…
Q: Where is the DNA that encodes the last 13 residues of the variable domain?
A: Antibodies are made up of four polypeptide chains namely two heavy chains and two light chains (L).…
Q: What is the unit of a genetic map?
A: Genetic map: Gene mapping as the name suggests that it is the map of genes that are present inside…
Q: What are WatsonCrick base pairs?
A: Nucleic acids are the organic materials present in all organisms in the form of DNA or RNA. These…
Q: Determine the 17 b p primers that can be used to locate the gene into which the transposon is…
A: The genes are a series of nucleotides found on chromosomes that code for a particular protein that…
Q: Why do trans-sRNAs often require a chaperone protein?
A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…
Q: How do we know if a genomic DNA sequence contains aprotein-coding gene?
A: Only about 1% of DNA contains protein-coding genes; the remaining 99 percent is noncoding. Noncoding…
Q: What is methylated cap?
A: Post-transcriptional modification includes a set of biological process occurring in eukaryotes by…
Q: Explain why it is sometimes difficult to locate genomic regions that encode a protein.
A: A gene is a DNA-based functional heredity unit that delivers instructions for the production of RNA…
Q: How can the number of repeats of a microsatellite/STR increase?
A: A repetitive DNA tract wherein a particular motif of DNA is repeated 5 to 50 times is termed a…
Q: What are gene coordinates?
A: Genes are the basic structural and functional units of heredity. They carry coded genetic…
Q: describe what is s autonomouslyreplicating sequences?
A: Yeast is eukaryotic, single-celled microorganism classified as members of the fungus kingdom. The…
Q: In the genome of Zea mays, in the segment 120M to 120,030k of Chromosome 5, what repeat elements do…
A: A monoecious plant has male and female flowers on the same plant, or flowers that have both male and…
Q: Describe the function of telomere repeat-binding factors.
A: Telomeres are the ends of chromosomes. they are made up of short repetitive sequences and prevent…
Q: What are the functions of the AT-rich region and DnaA boxes?
A: Bacterial replication starts at the unique site present in the chromosome known as oriC. The…
Q: What are the molecular components of degredasome?
A: The degradosome is a multiprotein complex present in most microscopic organisms that are associated…
Q: What factor might potentially determine the length of the flanking direct repeats that are produced…
A: Flanking or terminal repeats are the sequences DNA that are repeated on the both ends of a sequence…
Q: What are HU and H-NS (Histone-like Nucleoid Structuring) proteins ?
A: Chromatin is a substance found within chromosomes that is made up of DNA and protein. The DNA…
Q: What are the variable number of tandem repeats?
A: Variable number of tandem repeats (VNTR) are regions in the genome where a short nucleotide…
Q: • Inversions alter the order, but not the number, of geneson a __________
A: The number and the structure of the chromosomes are maintained as such during the meiosis, however,…
Q: What is a common proximal-promoter element ?
A: Carbohydrates, proteins, lipids, nucleic acids are the different macromolecules that are present in…
Q: What would be the minimum length(approximate number of bases) of an mRNAthat coded for a protein 300…
A: To determine: To determine the required minimum length of an mRNA that is coded for a protein of 300…
Q: what is Composite transposon
A: Transposons are the DNA sequences capable of changing its position or location within the genome of…
Q: The best molecular technique to quantify the gene transcripts is (write in fu!!).
A: The Central Dogma concept states that "DNA makes RNA and RNA makes protein". This concept is…
Q: where is the transcript headed following transcription and why?
A: Ad per the central dogma, the genetic information that is present in the coded form (DNA) is…
Q: What is the E-value of the sequence, what does your E-value reflect and what’s the importance of an…
A: Bioinformatics is the branch of science that deals with the genome, gene, and protein sequences. It…
What are the factor of expressed sequence tag?
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- What is the length in AA’s of the LilP protein? Assume fMet is NOT CLEAVED. Enter just the number, nothing else! Write out the sequence of the polypeptide in AA: use the three letter notation, e.g. Met-Ser-Pro- A lilP mutant called lilPXS is isolated that produces a truncated polypeptide of only 6 AA in length. Describe a single basepair DNA change that would lead to this truncated version of the protein. Multiple options are possible (100 words max.)How are the repeated sequences generated?You would like to add a nuclear localization sequence (NLS) of Lys-Lys-Lys-Arg-Lys to a protein that is usually found in the cytoplasm of a yeast cell. To accomplish this, you introduce the nucleotide sequence encoding the NLS into the gene that encodes the cytoplasmic protein of interest. a. What is the size of the nucleotide insert that will encode the NLS? Briefly explain. 5' 3' b. Below is a diagram of the gene encoding the cytoplasmic protein of interest in the yeast genome. If your goal is to put the NLS at the carboxyl (C) terminus of the protein, at which location (A-E) should the NLS be inserted? Briefly explain. A TATAA ATATT promoter +1 B ATG TAC D TAA ATT stop codon E 3' 5'
- What is a repetitive element in genomics? What are the types of repetitive elements? What is their effect on the ease of determining and analyzing a genome sequence?For the following sequence, what is the Tm? 5'-AGCTACGATCAGGTCA-3'The coding sequences of Gene F and Gene G are shown by the double-stranded DNA below. Using the genetic code, what is the polypeptide chain sequence when Gene G is completely expressed?