This shows where EcoR1 cuts, where HindIII cuts, and where BstEII cuts. Given that the entire chromosome is 48,502 base pairs (as it says), figure out the fragment sizes you will expect if you cut it with EcoR1, or with HindIII, or with BseEII. Draw a map like the example attached " Show the sizes of each piece. Color code it according to which enzyme cut it.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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This shows where EcoR1 cuts, where HindIII cuts, and where BstEII cuts. Given that the entire chromosome is 48,502 base pairs (as it says), figure out the fragment sizes you will expect if you cut it with EcoR1, or with HindIII, or with BseEII. Draw a map like the example attached " Show the sizes of each piece. Color code it according to which enzyme cut it.

Here is an example of the map you will make with the data on the back page of the Southern
blot procedure. Color code the enzymes and show the sizes of the pieces that will be produced.
Remember to include the final piece, which is the end of the DNA minus the last cut site.
EcoR1
Hindlll
BstEll
2716
17659
21,367
8.258
8,258
14,972
25,011
42,578
30,058
37,852
47,284 50,000
14972
15086
29,625
7794
12148
17567
25011
7422
This is the back page do the southern blot procedure.
Restriction site locations within the lambda chromosome
(48,502 base pairs)
EcoRI sites
21,226
26,104
31,747
39,168
44,972
Hindlll sites
23,130
25,157
27,479
36,895
37,459
37,584
44,141
BstEll sites
5687
7058
8322
9024
13,348
13,572
13,689
16,012
17,941
25,183
30,005
36,374
40,049
Transcribed Image Text:Here is an example of the map you will make with the data on the back page of the Southern blot procedure. Color code the enzymes and show the sizes of the pieces that will be produced. Remember to include the final piece, which is the end of the DNA minus the last cut site. EcoR1 Hindlll BstEll 2716 17659 21,367 8.258 8,258 14,972 25,011 42,578 30,058 37,852 47,284 50,000 14972 15086 29,625 7794 12148 17567 25011 7422 This is the back page do the southern blot procedure. Restriction site locations within the lambda chromosome (48,502 base pairs) EcoRI sites 21,226 26,104 31,747 39,168 44,972 Hindlll sites 23,130 25,157 27,479 36,895 37,459 37,584 44,141 BstEll sites 5687 7058 8322 9024 13,348 13,572 13,689 16,012 17,941 25,183 30,005 36,374 40,049
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