The horizontal line represents the whole genome of Lambda DNA (a virus that infects a bacterial cell). The genome is 48,502 base pairs long. You are going to estimate the number and length in base pairs the fragments that result from cutting the lambda genome with three different enzymes (Eco R1, Hind III and Bam H1) Keep in mind that you are working with a linearized lambda DNA (has ends) because the DNA has been heated to 65oC and therefore, the cos site is not intact (not annealed) ProcedureRestriction maps for Lambda DNALambda DNA may exist as a linear or circular molecule. The linear Lambda DNA is 48,502 basepairs in length. It contains five EcoR I restriction sites or cleavage sites. These restriction sites areillustrated below. Each number indicates the distance, in base pairs, that the restriction site is locatedfrom the end of the linear Lambda DNA molecule. Hind III recognizes seven. restriction sites.BamH I recognizes five sites. Figures 1–3 show the restriction sites for the enzymės we will use. Inthe spaces provided below each map, calculate the size, in base pairs, of the individual fragmentsproduced by each enzyme.26,10421,22631,74739,16844,97248,502Number of I DNA fragments produced by digestion with EcoR I:Size, in base pairs (bp), of fragments produced by EcoR I digestion:Figure 1: Restriction sites for ECORI27,47937,58423,13036,89544,14125,15737,45948,502Number of fragments produced:Size of fragments produced:Figure 2: Restriction sites for Hind II27,9475,50522,34634,49941,73248,502Number of fragments produced:Size of fragments produced:Figure 3: Restriction sites for BamHI

Answered: Procedure Restriction maps for Lambda…

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The horizontal line represents the whole genome of Lambda DNA (a virus that infects a bacterial cell). The genome is 48,502 base pairs long. You are going to estimate the number and length in base pairs the fragments that result from cutting the lambda genome with three different enzymes (Eco R1, Hind III and Bam H1)

Keep in mind that you are working with a linearized lambda DNA (has ends) because the DNA has been heated to 65^oC and therefore, the cos site is not intact (not annealed)

Procedure
Restriction maps for Lambda DNA
Lambda DNA may exist as a linear or circular molecule. The linear Lambda DNA is 48,502 base
pairs in length. It contains five EcoR I restriction sites or cleavage sites. These restriction sites are
illustrated below. Each number indicates the distance, in base pairs, that the restriction site is located
from the end of the linear Lambda DNA molecule. Hind III recognizes seven. restriction sites.
BamH I recognizes five sites. Figures 1–3 show the restriction sites for the enzymės we will use. In
the spaces provided below each map, calculate the size, in base pairs, of the individual fragments
produced by each enzyme.
26,104
21,226
31,747
39,168
44,972
48,502
Number of I DNA fragments produced by digestion with EcoR I:
Size, in base pairs (bp), of fragments produced by EcoR I digestion:
Figure 1: Restriction sites for ECORI
27,479
37,584
23,130
36,895
44,141
25,157
37,459
48,502
Number of fragments produced:
Size of fragments produced:
Figure 2: Restriction sites for Hind II
27,947
5,505
22,346
34,499
41,732
48,502
Number of fragments produced:
Size of fragments produced:
Figure 3: Restriction sites for BamHI

Expert Solution

Step 1

The restriction enzyme is the restriction endonuclease which recognises and cut at the specific sequence in either a staggered or blunt manner.

The given genome is of Lambda phage whose cos site is not intact. The cos sites help in the circularization of phage DNA when present. Due to the loss of cos site, the genome of lambda phage will remain linear and will not circularize.

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