Interpret the dideoxy chain-terminating polyacrylamide sequencing gel by assigning the individual nucleotide bases in order next to the DNA bands. +ddCTP +ddGTP +ddTTP +ddATP Incorrect Answer A T с A G T Answer Bank A C A T с T T с T G A G Feedback Sorry, that's incorrect. You have not correctly assigned nucleotide bases to all of the bands in the +ddCTP lane. When a small amount of ddCTP is added to an otherwise normal reaction system, the synthesized strands will be prematurely terminated at some locations where dC normally occurs. Thus, each DNA fragment in that sample will end with a C residue. +95 points still possible
Interpret the dideoxy chain-terminating polyacrylamide sequencing gel by assigning the individual nucleotide bases in order next to the DNA bands. +ddCTP +ddGTP +ddTTP +ddATP Incorrect Answer A T с A G T Answer Bank A C A T с T T с T G A G Feedback Sorry, that's incorrect. You have not correctly assigned nucleotide bases to all of the bands in the +ddCTP lane. When a small amount of ddCTP is added to an otherwise normal reaction system, the synthesized strands will be prematurely terminated at some locations where dC normally occurs. Thus, each DNA fragment in that sample will end with a C residue. +95 points still possible
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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