Interpret the dideoxy chain-terminating polyacrylamide sequencing gel by assigning the individual nucleotide bases in order next to the DNA bands. +ddCTP +ddGTP +ddTTP +ddATP Incorrect Answer A T с A G T Answer Bank A C A T с T T с T G A G Feedback Sorry, that's incorrect. You have not correctly assigned nucleotide bases to all of the bands in the +ddCTP lane. When a small amount of ddCTP is added to an otherwise normal reaction system, the synthesized strands will be prematurely terminated at some locations where dC normally occurs. Thus, each DNA fragment in that sample will end with a C residue. +95 points still possible

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
Question

please help

Interpret the dideoxy chain-terminating polyacrylamide sequencing gel by assigning the individual nucleotide bases in order
next to the DNA bands.
+ddCTP
+ddGTP
+ddTTP
+ddATP
Incorrect Answer
A
T
с
A
G
T
Answer Bank
A
C
A
T
с
T
T
с
T
G
A
G
Feedback
Sorry, that's incorrect.
You have not correctly assigned
nucleotide bases to all of the bands in
the +ddCTP lane.
When a small amount of ddCTP is
added to an otherwise normal reaction
system, the synthesized strands will be
prematurely terminated at some
locations where dC normally occurs.
Thus, each DNA fragment in that
sample will end with a C residue.
+95 points still possible
Transcribed Image Text:Interpret the dideoxy chain-terminating polyacrylamide sequencing gel by assigning the individual nucleotide bases in order next to the DNA bands. +ddCTP +ddGTP +ddTTP +ddATP Incorrect Answer A T с A G T Answer Bank A C A T с T T с T G A G Feedback Sorry, that's incorrect. You have not correctly assigned nucleotide bases to all of the bands in the +ddCTP lane. When a small amount of ddCTP is added to an otherwise normal reaction system, the synthesized strands will be prematurely terminated at some locations where dC normally occurs. Thus, each DNA fragment in that sample will end with a C residue. +95 points still possible
Expert Solution
steps

Step by step

Solved in 2 steps

Blurred answer
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON