The starting concentration of the lambda DNA is usually 250 ng/μl. Biology 6 B DNA Restriction Digests, Ligations, & Electrophoresis Here's how much DNA you should use for each reaction: Uncut: 0.2 μg DNA (A small amount because it will all be in one band on the gel; if there's too much, the band will be smeared.) Convert to μl: EcoRl-cut: 1.0 μg DNA. Convert to μul: HindIII-cut: 3.0 μg DNA. (A larger amount because you're going to save some for later.) Convert to μl: EcoRI & HindIII-cut: 1.0 μg DNA. Convert to μl:

The starting concentration of the lambda DNA is usually 250 ng/μl. Biology 6 B DNA Restriction Digests, Ligations, & Electrophoresis Here's how much DNA you should use for each reaction: Uncut: 0.2 μg DNA (A small amount because it will all be in one band on the gel; if there's too much, the band will be smeared.) Convert to μl: EcoRl-cut: 1.0 μg DNA. Convert to μul: HindIII-cut: 3.0 μg DNA. (A larger amount because you're going to save some for later.) Convert to μl: EcoRI & HindIII-cut: 1.0 μg DNA. Convert to μl:

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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