The starting concentration of the lambda DNA is usually 250 ng/μl. Biology 6 B DNA Restriction Digests, Ligations, & Electrophoresis Here's how much DNA you should use for each reaction: Uncut: 0.2 μg DNA (A small amount because it will all be in one band on the gel; if there's too much, the band will be smeared.) Convert to μl: EcoRl-cut: 1.0 μg DNA. Convert to μul: HindIII-cut: 3.0 μg DNA. (A larger amount because you're going to save some for later.) Convert to μl: EcoRI & HindIII-cut: 1.0 μg DNA. Convert to μl:

The starting concentration of the lambda DNA is usually 250 ng/μl. Biology 6 B DNA Restriction Digests, Ligations, & Electrophoresis Here's how much DNA you should use for each reaction: Uncut: 0.2 μg DNA (A small amount because it will all be in one band on the gel; if there's too much, the band will be smeared.) Convert to μl: EcoRl-cut: 1.0 μg DNA. Convert to μul: HindIII-cut: 3.0 μg DNA. (A larger amount because you're going to save some for later.) Convert to μl: EcoRI & HindIII-cut: 1.0 μg DNA. Convert to μl:

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Why is DNA evidence more useful as exclusionary evidence than for positive identification of a suspect?
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QUESTION 10 Select all of the following that apply to the tradeoff between transmission and virulence that applies to many diseases. The tradeoff between transmission and virulence means that diseases always evolve to become more virulent. If greater virulence limits transmission, that disease will likely evolve to become less virulent than it could be. While making more copies of itself can increase the likelihood of transmission occurring, too much replication of the disease can make the host so sick it won't leave the house and spread the disease. A strain of a disease that replicates enough to be transmitted, but not so much that the host gets too sick to move, will be favored by natural selection over strains that either make the host too sick or do not replicate enough to be transmitted. If a disease can spread without making its host sick (e.g. when the host is asymptomatic), then the tradeoff between transmission and virulence does not apply.
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