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Draw the double-stranded sequence of each fragment after cleavage showing any phosphates left on the ends.

Draw the double-stranded sequence of each fragment after cleavage showing any phosphates left on the ends.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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**DNA Cleavage by a Hypothetical Restriction Endonuclease** **Given the following double-stranded fragment of DNA:** 5'- ACTTGCAGGCCTTCGATCC -3' 3'- TGAACGTCCGGAAGCTAGG -5' A hypothetical restriction endonuclease recognizes a 6bp sequence with two-fold symmetry (typical for restriction enzymes) found in this fragment and catalyzes cleavage of this DNA on both strands between GG nucleotides within the recognition sequence. This nuclease exhibits b-type cleavage (atypical for restriction enzymes). Draw the double-stranded sequence of each fragment after cleavage showing any phosphates left on the ends.
Transcribed Image Text:**DNA Cleavage by a Hypothetical Restriction Endonuclease** **Given the following double-stranded fragment of DNA:** 5'- ACTTGCAGGCCTTCGATCC -3' 3'- TGAACGTCCGGAAGCTAGG -5' A hypothetical restriction endonuclease recognizes a 6bp sequence with two-fold symmetry (typical for restriction enzymes) found in this fragment and catalyzes cleavage of this DNA on both strands between GG nucleotides within the recognition sequence. This nuclease exhibits b-type cleavage (atypical for restriction enzymes). Draw the double-stranded sequence of each fragment after cleavage showing any phosphates left on the ends.
Expert Solution
Step 1
  • Restriction endonucleases cut double stranded DNA at specific site or near specific site and form smaller fragments of the DNA.
  • Restriction endonucleases gives cut within DNA by recognizing specific nucleotide sequences in DNA.
  • Restriction endonucleases cut DNA bonds between 5' phosphate of one nucleotide  and 3' OH of other nucleotide.
  • Most of endonucleases  recognizes palindromic sequences on ds DNA.
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