A geneticist is interested in determining the locations of methylated cytosines within a fragment of DNA. She treats some copies of the fragment with sodium bisulfite and leaves some copies untreated. She then sequences the treated and untreated copies of the fragment and obtains the following results. Give the original sequence of the DNA fragment and indicate the locations of methylated cytosines. Sequence without treatment: — AATTGCCCGATCGATTAAGCCA — Sequence with treatment: — AATTGTTTGATCGATTAAGCTA —

Human Anatomy & Physiology (11th Edition)
11th Edition
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Chapter1: The Human Body: An Orientation
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A geneticist is interested in determining the locations of methylated
cytosines within a fragment of DNA. She treats some copies of the
fragment with sodium bisulfite and leaves some copies untreated. She
then sequences the treated and untreated copies of the fragment and
obtains the following results. Give the original sequence of the DNA
fragment and indicate the locations of methylated cytosines.
Sequence without
treatment:
— AATTGCCCGATCGATTAAGCCA

Sequence with
treatment:
— AATTGTTTGATCGATTAAGCTA

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