Which RE’s from table below have a 5’ overhang? Which ones have a 3’ Overhang? AccI GT^CGAC BamHI G^GATCC ClaI AT^CGAT NsiI ATGCA^T PstI CTGCA^G BglII A^GATCT TaqI T^CGA
After restriction enzymes cut, they contain unpaired bases. Type II restriction enzymes leave ends that may be 5' overhanging, 3' overhanging, or blunt. In all cases each end is left with a 3' OH and a 5' phosphate. All blunt ends, and any complementary overhanging ends may be re-ligated with T4 DNA ligase, as long as at least one 5'- phosphate is present.
In the tables below G^AATTC means that the end after cutting with enzyme will be:
-----G 3'
-----CTTAA 5'
GTGCA^C means that the end will be:
-----GTGCA 3'
-----C 5'
Which RE’s from table below have a 5’ overhang? Which ones have a 3’ Overhang?
|
AccI |
GT^CGAC |
BamHI |
G^GATCC |
|
ClaI |
AT^CGAT |
NsiI |
ATGCA^T |
|
PstI |
CTGCA^G |
BglII |
A^GATCT |
|
TaqI |
T^CGA |
|
|
Restriction enzymes are the specific enzymes that recognize and cut at specific sites on nucleotide sequences known as "restriction sites".
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