When using the plasmid shown below for cloning, which restriction enzyme site(s) (all indicated with capital letters) would you generally NOT use? (Single letters are used to denote sites for various restriction enzymes.) B 7 kb OX 0 S 5 kb L E H S ON B X 10 kb ORI pcDNA3 plasmid 14 kb E-0 bases N-20 bases S-30 bases H - 40 bases S 1 kb
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
I know X and S are correct, but it's saying there is at least one more answer.
![**Plasmid Cloning Exercise**
**Question:**
When using the plasmid shown below for cloning, which restriction enzyme site(s) (all indicated with capital letters) would you generally **NOT** use? (Single letters are used to denote sites for various restriction enzymes.)
**Diagram Explanation:**
- **Plasmid Name:** pCDNA3 plasmid
- **Plasmid Size:** 14 kb
- **Features:**
- **AmpR:** Ampicillin resistance gene
- **ORI:** Origin of replication
- **Restriction Sites:**
- **E:** 0 bases
- **N:** 20 bases
- **S:** 30 bases
- **H:** 40 bases
**Plasmid Map:**
- The circular map illustrates the locations of different restriction sites and their relative distances in kilobases:
- **X:** 10 kb
- **B:** 7 kb
- **S:** 1 kb
- **S:** 5 kb
**Options:**
- [ ] X
- [ ] E
- [ ] H
- [ ] S
- [ ] N
- [ ] B
*Consideration:* Choose the enzyme site(s) you would generally avoid using in cloning based on their position and compatibility with the plasmid's features.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Faefc02fc-f1ae-4f90-a486-edd86b942252%2F65edcef0-bafd-4ba3-8985-98f28a6de0ed%2Fkjzh33q_processed.png&w=3840&q=75)
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