Assume complete digestion of a single linear piece of DNA digested (cut) with EcoRI and Hindill. Assume complete digestion of the plasmid digested with Eco and HindIll. What comes out of the plasmid when you digest with EcoRl and Hindill? What could ligate into the plasmid during ligation! Need help answering these questions

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Chapter1: The Human Body: An Orientation
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Assume complete digestion of a single linear piece of DNA digested (cut) with EcoRI and Hindill. Assume complete digestion of the plasmid digested with Eco and HindIll. What comes out of the plasmid when you digest with EcoRl and Hindill? What could ligate into the plasmid during ligation! Need help answering these questions
Linear DNA and the pUC plasmid were both digested with Hind III and EcoRI and then put
together in a ligation reaction. Assume complete digestion. What are the sizes of the 2 possible
ligation products? Show your work.
Hind II
EcoRI
BamH1
Xbol
150 bp
200 bp
400 bp
100 bp
200 bp
MCS:
EcoRI - 396
Sacl - 402
Amp
pUC
lacZ
Smal -412
2686 bp
Xbol - 423
Pstl - 435
Hindll -447
2. Following the ligation performed in #1 above, suppose that you next do a transformation
and plate the cells on media containing ampicillin and X-gal. Complete the following table to
describe your anticipated results.
Ampicillin
Production of
Color of cells
Cells
resistance?
B-galactosidase?
on the plate?
Transcribed Image Text:Linear DNA and the pUC plasmid were both digested with Hind III and EcoRI and then put together in a ligation reaction. Assume complete digestion. What are the sizes of the 2 possible ligation products? Show your work. Hind II EcoRI BamH1 Xbol 150 bp 200 bp 400 bp 100 bp 200 bp MCS: EcoRI - 396 Sacl - 402 Amp pUC lacZ Smal -412 2686 bp Xbol - 423 Pstl - 435 Hindll -447 2. Following the ligation performed in #1 above, suppose that you next do a transformation and plate the cells on media containing ampicillin and X-gal. Complete the following table to describe your anticipated results. Ampicillin Production of Color of cells Cells resistance? B-galactosidase? on the plate?
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