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Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy.   What is the largest fragment size that the BamHI/NcoI double digest produces?     2.250 kb     2.500 kb     2kb     750 b

Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy.   What is the largest fragment size that the BamHI/NcoI double digest produces?     2.250 kb     2.500 kb     2kb     750 b

Human Heredity: Principles and Issues (MindTap Course List)
Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter14: Biotechnology And Society
Section: Chapter Questions
Problem 12QP: DNA Profiles as Tools for Identification A PCR-based paternity test is conducted using STRs that...
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Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy.

 

What is the largest fragment size that the BamHI/NcoI double digest produces?

   

2.250 kb
   

2.500 kb
   

2kb
   

750 b
### Gel Electrophoresis Image Analysis This image represents a gel electrophoresis analysis, which is a technique used to separate DNA fragments based on their size. #### Lane Descriptions: - **L**: Ladder (L) - A standard DNA ladder used as a reference to estimate the size of DNA fragments. The bands are labeled with their respective base pair (bp) sizes: 3000 bp, 2000 bp, 1000 bp, 800 bp, 700 bp, and 500 bp. - **Uncut**: This lane contains uncut DNA, meaning the DNA has not been treated with any restriction enzymes. It serves as a control. - **EcoRI**: DNA sample treated with the EcoRI restriction enzyme. - **BamHI**: DNA sample treated with the BamHI restriction enzyme. - **NcoI**: DNA sample treated with the NcoI restriction enzyme. - **EcoRI/BamHI**: DNA sample treated with both EcoRI and BamHI restriction enzymes. - **BamHI/NcoI**: DNA sample treated with both BamHI and NcoI restriction enzymes. - **NcoI/EcoRI**: DNA sample treated with both NcoI and EcoRI restriction enzymes. - **B/N/E**: DNA sample treated with BamHI, NcoI, and EcoRI restriction enzymes. #### Analysis of DNA Fragments: - **Ladder (L)**: The sizes of the DNA fragments are used as markers. The most prominent bands correspond to 3000 bp, 2000 bp, 1000 bp, 800 bp, 700 bp, and 500 bp. - **Uncut lane**: The uncut DNA appears as one high-molecular-weight band, indicating that the DNA is intact and not fragmented. - **Single enzyme cuts (EcoRI, BamHI, NcoI)**: Each of these lanes shows a distinct pattern of bands, which corresponds to the fragment sizes generated by digestion with the respective restriction enzyme. - **Combination enzyme cuts (EcoRI/BamHI, BamHI/NcoI, NcoI/EcoRI)**: These lanes show multiple bands, indicating that the DNA has been cut at more than one site. The patterns are more complex and reveal the combined cutting sites of both enzymes used. - **Triple enzyme cut lane (B/N/E)**: This lane shows a distinct pattern of multiple bands indicating that the
Transcribed Image Text:### Gel Electrophoresis Image Analysis This image represents a gel electrophoresis analysis, which is a technique used to separate DNA fragments based on their size. #### Lane Descriptions: - **L**: Ladder (L) - A standard DNA ladder used as a reference to estimate the size of DNA fragments. The bands are labeled with their respective base pair (bp) sizes: 3000 bp, 2000 bp, 1000 bp, 800 bp, 700 bp, and 500 bp. - **Uncut**: This lane contains uncut DNA, meaning the DNA has not been treated with any restriction enzymes. It serves as a control. - **EcoRI**: DNA sample treated with the EcoRI restriction enzyme. - **BamHI**: DNA sample treated with the BamHI restriction enzyme. - **NcoI**: DNA sample treated with the NcoI restriction enzyme. - **EcoRI/BamHI**: DNA sample treated with both EcoRI and BamHI restriction enzymes. - **BamHI/NcoI**: DNA sample treated with both BamHI and NcoI restriction enzymes. - **NcoI/EcoRI**: DNA sample treated with both NcoI and EcoRI restriction enzymes. - **B/N/E**: DNA sample treated with BamHI, NcoI, and EcoRI restriction enzymes. #### Analysis of DNA Fragments: - **Ladder (L)**: The sizes of the DNA fragments are used as markers. The most prominent bands correspond to 3000 bp, 2000 bp, 1000 bp, 800 bp, 700 bp, and 500 bp. - **Uncut lane**: The uncut DNA appears as one high-molecular-weight band, indicating that the DNA is intact and not fragmented. - **Single enzyme cuts (EcoRI, BamHI, NcoI)**: Each of these lanes shows a distinct pattern of bands, which corresponds to the fragment sizes generated by digestion with the respective restriction enzyme. - **Combination enzyme cuts (EcoRI/BamHI, BamHI/NcoI, NcoI/EcoRI)**: These lanes show multiple bands, indicating that the DNA has been cut at more than one site. The patterns are more complex and reveal the combined cutting sites of both enzymes used. - **Triple enzyme cut lane (B/N/E)**: This lane shows a distinct pattern of multiple bands indicating that the
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