Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy. What is the largest fragment size that the BamHI/NcoI double digest produces? 2.250 kb 2.500 kb 2kb 750 b
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy.
What is the largest fragment size that the BamHI/NcoI double digest produces?
2.250 kb |
||
2.500 kb |
||
2kb |
||
750 b |
![### Gel Electrophoresis Image Analysis
This image represents a gel electrophoresis analysis, which is a technique used to separate DNA fragments based on their size.
#### Lane Descriptions:
- **L**: Ladder (L) - A standard DNA ladder used as a reference to estimate the size of DNA fragments. The bands are labeled with their respective base pair (bp) sizes: 3000 bp, 2000 bp, 1000 bp, 800 bp, 700 bp, and 500 bp.
- **Uncut**: This lane contains uncut DNA, meaning the DNA has not been treated with any restriction enzymes. It serves as a control.
- **EcoRI**: DNA sample treated with the EcoRI restriction enzyme.
- **BamHI**: DNA sample treated with the BamHI restriction enzyme.
- **NcoI**: DNA sample treated with the NcoI restriction enzyme.
- **EcoRI/BamHI**: DNA sample treated with both EcoRI and BamHI restriction enzymes.
- **BamHI/NcoI**: DNA sample treated with both BamHI and NcoI restriction enzymes.
- **NcoI/EcoRI**: DNA sample treated with both NcoI and EcoRI restriction enzymes.
- **B/N/E**: DNA sample treated with BamHI, NcoI, and EcoRI restriction enzymes.
#### Analysis of DNA Fragments:
- **Ladder (L)**: The sizes of the DNA fragments are used as markers. The most prominent bands correspond to 3000 bp, 2000 bp, 1000 bp, 800 bp, 700 bp, and 500 bp.
- **Uncut lane**: The uncut DNA appears as one high-molecular-weight band, indicating that the DNA is intact and not fragmented.
- **Single enzyme cuts (EcoRI, BamHI, NcoI)**: Each of these lanes shows a distinct pattern of bands, which corresponds to the fragment sizes generated by digestion with the respective restriction enzyme.
- **Combination enzyme cuts (EcoRI/BamHI, BamHI/NcoI, NcoI/EcoRI)**: These lanes show multiple bands, indicating that the DNA has been cut at more than one site. The patterns are more complex and reveal the combined cutting sites of both enzymes used.
- **Triple enzyme cut lane (B/N/E)**: This lane shows a distinct pattern of multiple bands indicating that the](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F7fa5fbc8-b7a6-472a-a4e2-348483b01488%2F3d2caf94-e39c-4882-a0b8-9a26cea0baf2%2Fduzrjue_processed.png&w=3840&q=75)
![](/static/compass_v2/shared-icons/check-mark.png)
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
![Blurred answer](/static/compass_v2/solution-images/blurred-answer.jpg)
![Human Anatomy & Physiology (11th Edition)](https://www.bartleby.com/isbn_cover_images/9780134580999/9780134580999_smallCoverImage.gif)
![Biology 2e](https://www.bartleby.com/isbn_cover_images/9781947172517/9781947172517_coverImage_Textbooks.gif)
![Anatomy & Physiology](https://www.bartleby.com/isbn_cover_images/9781259398629/9781259398629_smallCoverImage.gif)
![Human Anatomy & Physiology (11th Edition)](https://www.bartleby.com/isbn_cover_images/9780134580999/9780134580999_smallCoverImage.gif)
![Biology 2e](https://www.bartleby.com/isbn_cover_images/9781947172517/9781947172517_coverImage_Textbooks.gif)
![Anatomy & Physiology](https://www.bartleby.com/isbn_cover_images/9781259398629/9781259398629_smallCoverImage.gif)
![Molecular Biology of the Cell (Sixth Edition)](https://www.bartleby.com/isbn_cover_images/9780815344322/9780815344322_smallCoverImage.gif)
![Laboratory Manual For Human Anatomy & Physiology](https://www.bartleby.com/isbn_cover_images/9781260159363/9781260159363_smallCoverImage.gif)
![Inquiry Into Life (16th Edition)](https://www.bartleby.com/isbn_cover_images/9781260231700/9781260231700_smallCoverImage.gif)